Product Pathways - Innate Immunity
IFIT1 (D2X9Z) Rabbit mAb #14769
|14769S||100 µl ( 10 western blots )||￥3,250.00||现货查询 购买询价 防伪查询|
|14769||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),
Specificity / Sensitivity
IFIT1 (D2X9Z) Rabbit mAb recognizes endogenous levels of total IFIT1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human IFIT1 protein.
Western blot analysis of extracts from HeLa cells, untreated (-), or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml, 16 hr; +), using IFIT1 (D2X9Z) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-), or transfected with a construct expressing DDK-tagged full-length human IFIT1 (hIFIT1-DDK; +), using IFIT1 (D2X9Z) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with Human Interferon-alpha1 (hIFN-alpha1) #8927 (right) using IFIT1 (D2X9Z) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human spleen using IFIT1 (D2X9Z) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using IFIT1 (D2X9Z) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
IFIT1 (interferon-induced protein with tetratricopeptide repeats 1) belongs to the IFIT family of proteins, which consists of four members in humans (IFIT1, IFIT2, IFIT3, and IFIT5) and three members in mice (IFIT1, IFIT2, and IFIT3) (1). IFIT1 expression is induced by Type I Interferons resulting from viral infection (2). IFIT1 is an antiviral protein that directly binds viral RNA that has a 5’ triphosphate group (PPP-RNA) (2). In humans, the viral PPP-RNA bound IFIT1 forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent replication (2). IFIT1 has also been shown to inhibit translation by binding to the eukaryotic initiation factor-3 (eIF-3) (3).
- Ablasser, A. and Hornung, V. (2011) Nat Immunol 12, 588-90.
- Pichlmair, A. et al. (2011) Nat Immunol 12, 624-30.
- Guo, J. et al. (2000) EMBO J 19, 6891-9.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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