Cell Signaling Technology

Product Pathways - DNA Damage

XPC (D1M5Y) Rabbit mAb #14768


No. Size Price
14768S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14768 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 120 Rabbit IgG
IP 1:100
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

XPC (D1M5Y) Rabbit mAb recognizes endogenous levels of total XPC protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human XPC protein.



Confocal immunofluorescent analysis of MCF7 (left) and MDA-MB-231 (right) cells using (D1M5Y) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and MDA-MB-231 cells using XPC (D1M5Y) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).



Immunoprecipitation of XPC from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or XPC (D1M5Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using XPC (D1M5Y) Rabbit mAb.


Nucleotide excision repair (NER) is a process by which cells identify and repair DNA lesions resulting from chemical or radiation exposure (1). XPC forms a complex with HR23B (2) that acts as a damage sensor due to its high affinity for geometry distorting DNA lesions. This complex localizes to sites of DNA damage and recruits the remaining members of the preincision complex necessary for initiation of NER (3). XPC is one of eight NER proteins (XPA-G, XPV) where defects result in Xeroderma pigmentosum, a disease characterized by sunlight sensitivity, a predisposition to cancer of exposed tissue, and, in some instances, neurological defects (4).

  1. Fuss, J.O. and Cooper, P.K. (2006) PLoS Biol 4, e203.
  2. Masutani, C. et al. (1994) EMBO J 13, 1831-43.
  3. Sugasawa, K. et al. (1998) Mol Cell 2, 223-32.
  4. DiGiovanna, J.J. and Kraemer, K.H. (2012) J Invest Dermatol 132, 785-96.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :