Product Pathways - DNA Damage
XPC (D1M5Y) Rabbit mAb #14768
|14768S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|14768||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
XPC (D1M5Y) Rabbit mAb recognizes endogenous levels of total XPC protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human XPC protein.
Confocal immunofluorescent analysis of MCF7 (left) and MDA-MB-231 (right) cells using (D1M5Y) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).
Western blot analysis of extracts from MCF7 and MDA-MB-231 cells using XPC (D1M5Y) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Nucleotide excision repair (NER) is a process by which cells identify and repair DNA lesions resulting from chemical or radiation exposure (1). XPC forms a complex with HR23B (2) that acts as a damage sensor due to its high affinity for geometry distorting DNA lesions. This complex localizes to sites of DNA damage and recruits the remaining members of the preincision complex necessary for initiation of NER (3). XPC is one of eight NER proteins (XPA-G, XPV) where defects result in Xeroderma pigmentosum, a disease characterized by sunlight sensitivity, a predisposition to cancer of exposed tissue, and, in some instances, neurological defects (4).
- Fuss, J.O. and Cooper, P.K. (2006) PLoS Biol 4, e203.
- Masutani, C. et al. (1994) EMBO J 13, 1831-43.
- Sugasawa, K. et al. (1998) Mol Cell 2, 223-32.
- DiGiovanna, J.J. and Kraemer, K.H. (2012) J Invest Dermatol 132, 785-96.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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