Cell Signaling Technology

Product Pathways - Apoptosis

Noxa (D8L7U) Rabbit mAb #14766

No. Size Price
14766S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14766 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 10 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Noxa (D8L7U) Rabbit mAb recognizes endogenous levels of total Noxa protein. This antibody also cross-reacts with multiple unidentified proteins, most notably at 35, 50, and 80 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Noxa protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human Noxa protein (hNoxa; +), using Noxa (D8L7U) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved Jurkat cells, untreated (-) or treated with TPA #4174 (10 ng/ml, 3 hr; +), using Noxa (D8L7U) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from KARPAS-299, RL-7, and L-540 cells lines using Noxa (D8L7U) Rabbit mAb. Cell Line Source: Dr. Abraham Karpas at the University of Cambridge.

IP

IP

Immunoprecipitation of Noxa protein from KARPAS-299 cells using Rabbit (DA1E) mAb XP® Isotype Control #3900 (lane 2) or Noxa (D8L7U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Noxa (D8L7U) Rabbit mAb. Cell Line Source: Dr. Abraham Karpas at the University of Cambridge.

Background

Phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1, Noxa) is a small protein that plays a key role in mediating apoptotic signaling. Noxa is a pro-apoptotic Bcl-2 family protein that contains a single Bcl-2 homology (BH3) domain (1). Members of the “BH3-only” family (e.g. Noxa, Bad, Bim, Puma, Bid, Bik, and Hrk) are highly regulated proteins that induce apoptosis through BH3-dependent interaction with anti-apoptotic Bcl-2 family proteins (2). Noxa localizes to mitochondria and binds the anti-apoptotic proteins Mcl-1 and A1/Bfl-1, but does not bind to Bcl-2 or Bcl-xL (3). The Noxa protein competes with Mcl-1 for binding to mitochondrial Bak protein. Noxa was originally identified as a phorbol ester inducible protein that is highly expressed in adult T-cell leukemia cell lines (4). Several different stimuli, including DNA damage, hypoxia, interferon, viral infection, and double-stranded RNA, induce Noxa expression in cells. Higher levels of Noxa protein are typically found hematopoietic cells (3,5,6).

  1. Ploner, C. et al. (2008) Oncogene 27 Suppl 1, S84-92.
  2. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.
  3. Oda, E. et al. (2000) Science 288, 1053-8.
  4. Hijikata, M. et al. (1990) J Virol 64, 4632-9.
  5. Kim, J.Y. et al. (2004) J Exp Med 199, 113-24.
  6. Sun, Y. and Leaman, D.W. (2005) J Biol Chem 280, 15561-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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