Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

RTF1 (D7V3W) Rabbit mAb #14737

No. Size Price
14737S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14737 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 100 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, S. cerevisiae,

Specificity / Sensitivity

RTF1 (D7V3W) Rabbit mAb recognizes endogenous levels of total RTF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu364 of human RTF1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RTF1 (D7V3W) Rabbit mAb.

Background

The PAF (RNA polymerase II (RNAPII) associated factor) complex was initially identified in yeast and is comprised of subunits PAF1, Leo1, Ctr9, Cdc73, RTF1 and Ski8 (1,2). The PAF complex plays an important role in transcription initiation and elongation by RNAPII by regulating the establishment of proper histone modifications such as histone H2B ubiquitination and the recruitment of the histone chaperone FACT (facilitates chromatin transcription) (3-5). The PAF complex also plays a role in mRNA processing and maturation by interacting with and recruiting the cleavage and polyadenylation specificity factor and cleavage stimulation factor complexes via the Cdc73 subunit (6,7). In addition, the Ski8 subunit of the PAF complex is part of the hSKi complex that regulates RNA surveillance, suggesting an important function of the complex in coordinating events associated with proper RNA maturation during transcription (1,8).

The RNA polymerase-associated protein RTF1 and the tumor suppressor Cdc73 are responsible for recruitment of the PAF complex to active genes within chromatin (9). The RTF1 protein contains a pair of functional domains: the central plus 3 domain has been identified as a region of ssDNA binding (10), and a smaller histone modification domain (HMD) appears to promote methylation of the histone H3 and ubiquitination of histone H2B (9,11).

  1. Jaehning, J.A. (2010) Biochim Biophys Acta 1799, 379-88.
  2. Chaudhary, K. et al. (2007) Oncogene 26, 7499-507.
  3. Pavri, R. et al. (2006) Cell 125, 703-17.
  4. Rozenblatt-Rosen, O. et al. (2005) Mol Cell Biol 25, 612-20.
  5. Zhu, B. et al. (2005) Genes Dev 19, 1668-73.
  6. Rozenblatt-Rosen, O. et al. (2009) Proc Natl Acad Sci U S A 106, 755-60.
  7. Farber, L.J. et al. (2010) Mol Carcinog 49, 215-23.
  8. Warner, M.H. et al. (2007) Mol Cell Biol 27, 6103-15.
  9. de Jong, R.N. et al. (2008) Structure 16, 149-59.
  10. Piro, A.S. et al. (2012) Proc Natl Acad Sci U S A 109, 10837-42.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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