Product Pathways - Phosphatases
PTP-PEST (D4W7W) Rabbit mAb #14735
|14735S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|14735||carrier free & custom formulation / quantity||email request|
|W||1:1000||Human,Mouse,Rat,||Endogenous||110 to 125||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
PTP-PEST (D4W7W) Rabbit mAb recognizes endogenous levels of total PTP-PEST protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg747 of human PTP-PEST protein.
Western blot analysis of extracts from various cell lines using PTP-PEST (D4W7W) Rabbit mAb.
PTP-PEST is a ubiquitously expressed cytosolic protein tyrosine phosphatase with multiple proline-rich regions that appear to be the docking sites for PTP-PEST binding partners or substrates (1). PTP-PEST regulates fibroblast adhesion, migration, and cytokinesis through its association with and dephosphorylation of p130 Cas, paxillin, PSTPIP1, WASP, and other adhesion molecules (1-5). By modulating phosphorylation states of Shc, Pyk2, Fak, and WASP, PTP-PEST negatively regulates lymphocyte activation (1,6). In mammary epithelial cells, EGF facilitates the dephosphorylation of Jak2 by PTP-PEST, thereby interfering with lactogenic hormone PRL signaling (7). PTP-PEST dephosphorylates c-Abl as well, which affects the phosphorylation states of PTP-PEST substrates such as paxillin, p130 Cas, Crk, and PSTPIP1 (8).
PTP-PEST regulates adhesion and motility of cultured epithelial cells through modulation of Rho GTPase activity (9), and is required for integrin-mediated endothelial cell adhesion and migration (10).
- Davidson, D. and Veillette, A. (2001) EMBO J 20, 3414-26.
- Garton, A.J. and Tonks, N.K. (1999) J Biol Chem 274, 3811-8.
- Shen, Y. et al. (2000) J Biol Chem 275, 1405-13.
- Angers-Loustau, A. et al. (1999) J Cell Biol 144, 1019-31.
- Côté, J.F. et al. (2002) J Biol Chem 277, 2973-86.
- Badour, K. et al. (2004) J Exp Med 199, 99-112.
- Horsch, K. et al. (2001) Mol Endocrinol 15, 2182-96.
- Cong, F. et al. (2000) Mol Cell 6, 1413-23.
- Espejo, R. et al. (2010) Am J Physiol Cell Physiol 299, C454-63.
- Souza, C.M. et al. (2012) J Biol Chem 287, 43180-90.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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