Cell Signaling Technology

Product Pathways - Phosphatases

PTP-PEST (D4W7W) Rabbit mAb #14735

No. Size Price
14735S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14735 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 110 to 125 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PTP-PEST (D4W7W) Rabbit mAb recognizes endogenous levels of total PTP-PEST protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg747 of human PTP-PEST protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PTP-PEST (D4W7W) Rabbit mAb.



Immunoprecipitation of PTP-PEST from PANC-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PTP-PEST (D4W7W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using PTP-PEST (D4W7W) Rabbit mAb.


PTP-PEST is a ubiquitously expressed cytosolic protein tyrosine phosphatase with multiple proline-rich regions that appear to be the docking sites for PTP-PEST binding partners or substrates (1). PTP-PEST regulates fibroblast adhesion, migration, and cytokinesis through its association with and dephosphorylation of p130 Cas, paxillin, PSTPIP1, WASP, and other adhesion molecules (1-5). By modulating phosphorylation states of Shc, Pyk2, Fak, and WASP, PTP-PEST negatively regulates lymphocyte activation (1,6). In mammary epithelial cells, EGF facilitates the dephosphorylation of Jak2 by PTP-PEST, thereby interfering with lactogenic hormone PRL signaling (7). PTP-PEST dephosphorylates c-Abl as well, which affects the phosphorylation states of PTP-PEST substrates such as paxillin, p130 Cas, Crk, and PSTPIP1 (8).

PTP-PEST regulates adhesion and motility of cultured epithelial cells through modulation of Rho GTPase activity (9), and is required for integrin-mediated endothelial cell adhesion and migration (10).

  1. Davidson, D. and Veillette, A. (2001) EMBO J 20, 3414-26.
  2. Garton, A.J. and Tonks, N.K. (1999) J Biol Chem 274, 3811-8.
  3. Shen, Y. et al. (2000) J Biol Chem 275, 1405-13.
  4. Angers-Loustau, A. et al. (1999) J Cell Biol 144, 1019-31.
  5. Côté, J.F. et al. (2002) J Biol Chem 277, 2973-86.
  6. Badour, K. et al. (2004) J Exp Med 199, 99-112.
  7. Horsch, K. et al. (2001) Mol Endocrinol 15, 2182-96.
  8. Cong, F. et al. (2000) Mol Cell 6, 1413-23.
  9. Espejo, R. et al. (2010) Am J Physiol Cell Physiol 299, C454-63.
  10. Souza, C.M. et al. (2012) J Biol Chem 287, 43180-90.

Application References

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