Cell Signaling Technology

Product Pathways - Metabolism

CYP1A2 (D2V7S) Mouse mAb #14719

aryl hydrocarbon hydroxylase   CP12   CP1A2   CYP1A2   CYPIA2   Cytochrome P(3)450   Cytochrome P450 1A2   Cytochrome P450 4   Cytochrome P450-P3   cytochrome P450. family 1. subfamily A. polypeptide 2   cytochrome P450. subfamily I (aromatic compound-inducible). polypeptide 2   dioxin-inducible P3-450   flavoprotein-linked monooxygenase   microsomal monooxygenase   P3-450   P450 form 4   P450(PA)  

No. Size Price
14719S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
14719 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 55 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CYP1A2 (D2V7S) Mouse mAb recognizes endogenous levels of total CYP1A2 protein. This antibody does not cross-react with CYP1A1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with 3-methylcholanthrene-induced rat liver microsomal fractions.

Western Blotting

Western Blotting

Western blot analysis of extracts from human and rat livers using CYP1A2 (D2V7S) Mouse mAb.


Cytochrome P450 (CYP) is a family of enzymes that contain a heme group (1). These enzymes, when reduced and bound by carbon monoxide, maximally absorb light of 450 nm (1). Type I cytochrome P450s are found in mitochondria and function in the biosynthesis of essential molecules (1). Type II cytochrome P450s are found in endoplasmic reticulum (1). Some type II cytochrome P450s play a role in the biosynthesis of essential molecules whereas others metabolize xenobiotics (1). Research studies show that cytochrome P450s form various heteromeric complexes with other members of the P450 family influencing their catalytic activities (2-4). CYP1A2 is in the endoplasmic reticulum of hepatocytes and responsible for the breakdown of a variety of xenobiotic substances and bioactivation of carcinogens (2, 5). CYP1 enzymes, including CYP1A2, have been implicated in smoking-related osteoporosis (6). A meta-analysis shows that a particular polymorphism in CYP1A2 is potentially linked to increased cancer risk (5).

  1. Miller, W.L. (2012) Sci Signal 5, pt11.
  2. Backes, W.L. and Kelley, R.W. (2003) Pharmacol Ther 98, 221-33.
  3. Reed, J.R. et al. (2010) J Biol Chem 285, 8942-52.
  4. Kelley, R.W. et al. (2006) Biochemistry 45, 15807-16.
  5. Wang, H. et al. (2012) BMC Cancer 12, 528.
  6. Iqbal, J. et al. (2013) Proc Natl Acad Sci U S A 110, 11115-20.

Application References

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