Product Pathways - PI3K / Akt Signaling
Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb #14716
JC310 MAPKAP1 MEKK2-interacting protein 1 MGC2745 MIP1 Mitogen-activated protein kinase 2-associated protein 1 mitogen-activated protein kinase associated protein 1 mSIN1 ras inhibitor MGC2745 SAPK-interacting protein 1 SIN1 SIN1b SIN1g stress-activated map kinase interacting protein 1 Stress-activated map kinase-interacting protein 1 stress-activated protein kinase-interacting 1 Target of rapamycin complex 2 subunit MAPKAP1 TORC2 subunit MAPKAP1
|14716S||100 µl ( 10 western blots )||￥3,900.00 现货查询||购买询价|
|14716||carrier free & custom formulation / quantity||email request|
|W||1:1000||Human,Mouse,||Endogenous||78, 74||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Species predicted to react based on 100% sequence homology: Rat,
Specificity / Sensitivity
Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb recognizes endogenous levels of Sin1 protein only when phosphorylated at Thr86.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding phosphorylated Thr86 of human Sin1 protein.
Western blot analysis of extracts from serum starved NIH/3T3 cells, untreated (-) or treated with insulin (150 nM, 5 min; +) or Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 ng/ml, 5 min; +) in the absence (-) or presence (+) of λ phosphatase, using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from serum starved HeLa cells, untreated (-) or treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 ng/ml, 5 min; +) in the absence (-) or presence (+) of λ phosphatase, using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (upper) and Sin1 (D7G1A) Rabbit mAb #12860 (lower).
Immunoprecipitation of phospho-Sin1 (Thr86) from serum starved NIH/3T3 cell extracts treated with insulin (150 nM, 5 min) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb.
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase that is a component of two different complexes. The mTOR complex 1 (mTORC1), a target of rapamycin, contains mTOR, GβL, and raptor. mTORC2, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTORC2 complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). mTORC2 has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). In addition, phosphorylation of Sin1 at Thr86 by Akt/PKB was shown to regulate the activity of mTORC2 in adipocytes upon stimulation by growth factors (5).
- Sarbassov, D.D. et al. (2004) Curr Biol 14, 1296-302.
- Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
- Hresko, R.C. and Mueckler, M. (2005) J Biol Chem 280, 40406-16.
- Ali, S.M. and Sabatini, D.M. (2005) J Biol Chem 280, 19445-8.
- Humphrey, S.J. et al. (2013) Cell Metab 17, 1009-20.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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