Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb #14716

JC310   MAPKAP1   MEKK2-interacting protein 1   MGC2745   MIP1   Mitogen-activated protein kinase 2-associated protein 1   mitogen-activated protein kinase associated protein 1   mSIN1   ras inhibitor MGC2745   SAPK-interacting protein 1   SIN1   SIN1b   SIN1g   stress-activated map kinase interacting protein 1   Stress-activated map kinase-interacting protein 1   stress-activated protein kinase-interacting 1   Target of rapamycin complex 2 subunit MAPKAP1   TORC2 subunit MAPKAP1  

No. Size Price
14716S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
14716 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 78, 74 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb recognizes endogenous levels of Sin1 protein only when phosphorylated at Thr86.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding phosphorylated Thr86 of human Sin1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved NIH/3T3 cells, untreated (-) or treated with insulin (150 nM, 5 min; +) or Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 ng/ml, 5 min; +) in the absence (-) or presence (+) of λ phosphatase, using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved HeLa cells, untreated (-) or treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 ng/ml, 5 min; +) in the absence (-) or presence (+) of λ phosphatase, using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (upper) and Sin1 (D7G1A) Rabbit mAb #12860 (lower).

IP

IP

Immunoprecipitation of phospho-Sin1 (Thr86) from serum starved NIH/3T3 cell extracts treated with insulin (150 nM, 5 min) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb.

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase that is a component of two different complexes. The mTOR complex 1 (mTORC1), a target of rapamycin, contains mTOR, GβL, and raptor. mTORC2, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTORC2 complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). mTORC2 has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). In addition, phosphorylation of Sin1 at Thr86 by Akt/PKB was shown to regulate the activity of mTORC2 in adipocytes upon stimulation by growth factors (5).

  1. Sarbassov, D.D. et al. (2004) Curr Biol 14, 1296-302.
  2. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
  3. Hresko, R.C. and Mueckler, M. (2005) J Biol Chem 280, 40406-16.
  4. Ali, S.M. and Sabatini, D.M. (2005) J Biol Chem 280, 19445-8.
  5. Humphrey, S.J. et al. (2013) Cell Metab 17, 1009-20.

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