Cell Signaling Technology

Product Pathways - Metabolism

TXNIP (D5F3E) Rabbit mAb #14715

EST01027   HHCPA78   sc-271238   sc-33099   THIF   thioredoxin binding protein 2   thioredoxin interacting protein   Thioredoxin-binding protein 2   Thioredoxin-interacting protein   TXNIP   upregulated by 1.25-dihydroxyvitamin D-3   VDUP1   Vitamin D3 up-regulated protein 1  

No. Size Price
14715S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14715 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 55 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

TXNIP (D5F3E) Rabbit mAb recognizes endogenous levels of total TXNIP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val337 of human TXNIP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TXNIP (D5F3E) Rabbit mAb.

IP

IP

Immunoprecipitation of TXNIP from RL-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TXNIP (D5F3E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TXNIP (D5F3E) Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 was used as the secondary antibody.

Background

The ubiquitously expressed thioredoxin-interacting protein (TXNIP) binds and inhibits thioredoxin to regulate cellular redox state (1-3). Research studies demonstrate that hyperglycemia induces TXNIP expression and increases cellular oxidative stress (1). In addition, these studies show that TXNIP reduces glucose uptake directly by binding the glucose transporter Glut1 to stimulate receptor internalization or indirectly by reducing Glut1 mRNA levels (3). Additional studies indicate that TXNIP plays a role in the regulation of insulin mRNA transcription (4). Microarray analyses indicate that TXNIP acts downstream of PPARγ and is a putative tumor suppressor that may control thyroid cancer cell progression (5). In addition, the TXNIP protein may be a potential therapeutic target for the treatment of type 2 diabetes and some disorders related to ER-stress (6).

  1. Schulze, P.C. et al. (2004) J Biol Chem 279, 30369-74.
  2. Saxena, G. et al. (2010) J Biol Chem 285, 3997-4005.
  3. Wu, N. et al. (2013) Mol Cell 49, 1167-75.
  4. Xu, G. et al. (2013) Nat Med 19, 1141-6.
  5. Morrison, J.A. et al. (2014) Mol Cancer 13, 62.
  6. Robinson, K.A. et al. (2013) J Mol Endocrinol 50, 59-71.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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