Product Pathways - Development
YAP (D8H1X) XP® Rabbit mAb (PE Conjugate) #14710
|14710S||100 µl ( 50 tests )||￥4,264.00||现货查询 购买询价 防伪查询|
|14710||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Species predicted to react based on 100% sequence homology: Bovine, Guinea Pig, Horse,
Specificity / Sensitivity
YAP (D8H1X) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total YAP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human YAP protein. The epitope corresponds to a region surrounding Pro435 of human YAP isoform 1. This sequence region is 100% conserved among all known isoforms of human YAP protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated YAP (D8H1X) XP® Rabbit mAb #14074.
YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Upon phosphorylation at Ser127 by LATS1/2 kinases, YAP translocates to the cytoplasm, where it is sequestered through association with 14-3-3 proteins in an Akt-dependent manner (7-9).
- Sudol, M. (1994) Oncogene 9, 2145-52.
- Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
- Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
- Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
- Yagi, R. et al. (1999) EMBO J 18, 2551-62.
- Basu, S. et al. (2003) Mol Cell 11, 11-23.
- Zhao, B. et al. (2010) Genes Dev 24, 862-74.
- Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
- Yu, F.X. et al. (2012) Cell 150, 780-91.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
用户评论 --- 共 0 条