Cell Signaling Technology

Product Pathways - Companion Products

Anti-Rabbit IgG (H+L), (PE Conjugate) #14705

alexa   chain   conjugate   flow   heavy   igg   light   PE   phyco   rabbit   Secondary  

Applications Species-Reactivity Sensitivity MW (kDa) Isotype
F Goat

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Anti-Rabbit IgG (H+L), (PE Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected.

Less than 1% cross-reactivity is observed with immunoglobulins from other species.

Description

Affinity purified goat anti-rabbit IgG (H+L) antibody is conjugated to R-phycoerythrin. This product has been optimized for use as a secondary antibody in flow applications. Excitation is 450-580 nm and peak emission is 590 nm.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060. Anti-Rabbit IgG (H+L), (PE Conjugate) was used as a secondary antibody.

Directions for Use

The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.

Background

This product has been optimized for use as a secondary antibody in flow cytometry applications. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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