Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Ret (E1N9A) Rabbit mAb (Flow Preferred) #14699


No. Size Price
14699S 100 µl ( 400 tests ) ¥3,100.00 现货查询 购买询价
14699 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 150, 175 Rabbit IgG
IP 1:50
F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry,

Specificity / Sensitivity

Ret (E1N9A) Rabbit mAb recognizes endogenous levels of total Ret protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro320 of human Ret protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of TT cells (green) and HeLa cells (blue) using Ret (E1N9A) Rabbit mAb (Flow Preferred). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ret (E1N9A) (Flow Preferred) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicomponent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotrophic factor (GDNF) and its congeners neurturin, persephin, and artemin (2-4). Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity (5).

  1. Airaksinen, M.S. et al. (1999) Mol Cell Neurosci 13, 313-25.
  2. Takahashi, M. et al. (1989) Oncogene 4, 805-6.
  3. Manié, S. et al. (2001) Trends Genet 17, 580-9.
  4. Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54.
  5. Iwashita, T. et al. (1999) Oncogene 18, 3919-22.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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