Cell Signaling Technology

Product Pathways - Adhesion

VCAM-1 Antibody (Rodent Specific) #14694

sc-1504   sc-8304  

No. Size Price
14694S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14694 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,Rat, Endogenous 35-45, 85-100 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

VCAM-1 Antibody (Rodent Specific) recognizes endogenous levels of total VCAM-1 protein. This antibody recognizes both long and short isoforms of VCAM-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln203 of mouse VCAM-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MS-1 cells, untreated (-) or treated with Mouse Tumor Necrosis Factor-α (mTNF-α) #5178 (10 ng/ml, 16 hr; +), using VCAM-1 Antibody (Rodent Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 3T3, C2C12, and YB2/0 cells using VCAM-1 Antibody (Rodent Specific).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse VCAM-1 protein (mVCAM-1-Myc/DDK; +), using VCAM-1 Antibody (Rodent Specific).

Background

VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3).

  1. Osborn, L. et al. (1989) Cell 59, 1203-11.
  2. Hession, C. et al. (1991) J Biol Chem 266, 6682-5.
  3. Elices, M.J. et al. (1990) Cell 60, 577-84.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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