Cell Signaling Technology

Product Pathways - Vesicle Trafficking

Sec24D (D9M7L) Rabbit mAb #14687

No. Size Price
14687S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14687 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 115 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Sec24D (D9M7L) Rabbit mAb recognizes endogenous levels of total Sec24D protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro80 of human Sec24D protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Sec24D (D9M7L) Rabbit mAb.



Immunoprecipitation of Sec24D from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Sec24D (D9M7L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Sec24D (D9M7L) Rabbit mAb.


Coat Protein Complex II (COPII) is composed of five cytosolic proteins: Sec23/24 complex, Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex, thereby forming a prebudding complex that directly binds target molecules (1-3). The prebudding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec24 subunit of COPII coat is thought to play a critical role in cargo selection (2,6). It binds directly to cargo proteins at the ER and brings them to COPII vesicles through interaction with Sec23. There are four Sec24 isoforms in human cells: Sec24A, Sec24B, Sec24C, and Sec24D (7). In mice, mutations in Sec24B have been linked to developmental defects (8,9).

  1. Aridor, M. et al. (1998) J Cell Biol 141, 61-70.
  2. Miller, E.A. et al. (2003) Cell 114, 497-509.
  3. Mossessova, E. et al. (2003) Cell 114, 483-95.
  4. Barlowe, C. et al. (1994) Cell 77, 895-907.
  5. Bi, X. et al. (2007) Dev Cell 13, 635-45.
  6. Miller, E. et al. (2002) EMBO J 21, 6105-13.
  7. Tang, B.L. et al. (1999) Biochem Biophys Res Commun 258, 679-84.
  8. Merte, J. et al. (2010) Nat Cell Biol 12, 41-6; sup pp 1-8.
  9. Wansleeben, C. et al. (2010) Development 137, 1067-73.

Application References

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