Product Pathways - Metabolism
MRP1/ABCC1 (D7O8N) Rabbit mAb #14685
|14685S||100 µl ( 10 western blots )||￥3,250.00||现货查询 购买询价 防伪查询|
|14685||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
MRP1/ABCC1 (D7O8N) Rabbit mAb recognizes endogenous levels of total MRP1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val273 of human ABCC1 protein.
Confocal immunofluorescent analysis of Hep G2 (left) and SK-MEL-2 (right) cells using MRP1/ABCC1 (D7O8N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13504 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from A-549, PANC-1, and Hep G2 cells using MRP1/ABCC1 (D7O8N) Rabbit mAb.
Immunoprecipitation of MRP1/ABCC1 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MRP1/ABCC1 (D7O8N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MRP1/ABCC1 (D7O8N) Rabbit mAb.
Multidrug resistance-associated protein 1 (MRP1/ABCC1) is a member of the MRP subfamily of ATP-binding cassette (ABC) transporters (1). MRP1/ABCC1 protein functions as an organic anion transporter. It has a broad range of substrates, including antineoplastic or therapeutic agents and the glutathione (GSH) conjugates of these compounds. MRP1/ABCC1 also transports physiological substrates such as folates, GSH and GSH disulfide (GSSG) conjugates of steroids, leukotrienes, and prostaglandins (2,3).
Although MRP1/ABCC1 is generally expressed in normal tissue, upregulation of MRP1/ABCC1 has been found in a variety of solid tumors, including small cell lung cancer, breast cancer, and prostate cancer (1,4,5). Research studies show that overexpression of MRP1/ABCC1 facilitates the elimination of therapeutic agents from cancer cells and confers drug resistance in those patients. Research studies also show that elevated expression of MRP1/ABCC1 is a negative prognostic marker for breast cancer and small cell lung cancer, as the level of MRP1/ABCC1 is predictive of the response and toxicity of chemotherapeutic agents in those patients (6-10).
- Cole, S.P. et al. (1992) Science 258, 1650-4.
- Pajic, M. et al. (2005) Cancer Lett 228, 241-6.
- Deeley, R.G. and Cole, S.P. (2006) FEBS Lett 580, 1103-11.
- Atalay, C. et al. (2006) Tumour Biol 27, 309-18.
- Sánchez, C. et al. (2011) Prostate 71, 1810-7.
- Nooter, K. et al. (1997) Br J Cancer 76, 486-93.
- Hsia, T.C. et al. (2002) Lung 180, 173-9.
- Kuo, T.H. et al. (2003) Nucl Med Biol 30, 627-32.
- Sánchez, C. et al. (2009) Prostate 69, 1448-59.
- Vulsteke, C. et al. (2013) Ann Oncol 24, 1513-25.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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