Cell Signaling Technology

Product Pathways - Tag Antibodies

Digoxigenin (D8Q9J) Rabbit mAb #14682

Digoxigenin   Digoxin   hapten  

No. Size Price
14682S 100 µl ( 200 western blots ) ¥3,100.00 现货查询 购买询价
14682 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:20000 Transfected Only Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Digoxigenin (D8Q9J) Rabbit mAb recognizes digoxigenin conjugated molecules.

Source / Purification

Monoclonal antibody is produced by immunizing animals with KLH-conjugated digoxigenin.

Western Blotting

Western Blotting

Western blot analysis of digoxigenin-conjugated IgG (IgG/DIG, 100 ng; +) and digoxigenin-conjugated BSA (BSA/DIG, 0.1 ng; +) using Digoxigenin (D8Q9J) Rabbit mAb.

Background

Digoxigenin (DIG) is a highly immunogenic steroid derived from “foxglove” plants of the genus Digitalis (1). This small hapten molecule can be covalently linked to proteins, nucleic acids, compounds, and glycans. Antibodies targeted against the DIG hapten display high binding affinity to DIG-conjugated molecules and can be used in molecular biology assays, including in situ hybridization, western blot, ELISA, and TUNEL assays (2-4).

  1. Cardwell, H.M. and Smith, S. (1953) Experientia 9, 367-8.
  2. Kessler, C. (1991) Mol Cell Probes 5, 161-205.
  3. Tian, F. et al. (2010) Autophagy 6, 1107-14.
  4. Lin, L. et al. (2011) J Virol Methods 171, 91-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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