Cell Signaling Technology

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Dinitrophenol (D1D6) Rabbit mAb #14681

No. Size Price
14681S 100 µl ( 100 assays ) ¥3,100.00 现货查询 购买询价
14681 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:10000 All Species Expected, Transfected Only Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Dinitrophenol (D1D6) Rabbit mAb recognizes dinitrophenol-conjugated molecules.

Source / Purification

Monoclonal antibody is produced by immunizing animals with KLH-conjugated dinitrophenol.

Western Blotting

Western Blotting

Western blot analysis of dinitrophenol-conjugated IgG (IgG/DNP, 200 ng; +) and dinitrophenol-conjugated BSA (BSA/DNP, 0.001 ng; +) using Dinitrophenol (D1D6) Rabbit mAb.

Background

The synthetic compound dinitrophenol (DNP) is a small hapten molecule that can be conjugated to proteins for use in immunoassays (1). Antibodies that recognize dinitrophenol will display strong binding affinity to DNP conjugated molecules, including nucleic acids and proteins. These DNP-conjugated compounds can be used in in situ hybridization, western, northern, and Southern blotting (1,2). Dinitrophenol is a commonly used insecticide and has been used as a treatment for weight loss (3).

  1. Grzybowski, J. et al. (1993) Nucleic Acids Res 21, 1705-12.
  2. Lehtovaara, P. et al. (1993) PCR Methods Appl 3, 169-75.
  3. Goldgof, M. et al. (2014) J Biol Chem 289, 19341-50.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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