Product Pathways - Tyrosine Kinase / Adaptors
Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb #14678
|14678S||100 µl ( 10 western blots )||￥3,900.00 现货查询||购买询价|
|14678||carrier free & custom formulation / quantity||email request|
|W||1:1000||Human,||Endogenous||80 (NPM-ALK), 220 (ALK)||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),
Species predicted to react based on 100% sequence homology: Mouse, Rat,
Specificity / Sensitivity
Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb recognizes endogenous levels of ALK protein only when phosphorylated at Tyr1507 (equivalent to Tyr567 of NPM-ALK).
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1507 of human ALK protein.
Confocal immunofluorescent analysis of KARPAS-299 cells, untreated (left) or treated with Crizotinib #4401 (1 μM, 2hr; center), and HeLa cells (right), using Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
Flow cytometric analysis of KARPAS-299 cells, untreated (green) or treated with Crizotinib #4401 (1 μM, 2 hr, 37ºC; blue), using Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
Western blot analysis of extracts from KARPAS-299 cells, untreated (-) or treated with Crizotinib #4401 (1 μM, indicated times), using Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb (upper) and ALK (C26G7) Rabbit mAb #3333 (lower). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
Immunoprecipitation of phospho-ALK from KARPAS-299 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb. Western blot analysis was performed using Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb. KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5).
A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8).
Phosphorylation of ALK on Tyr1507 was identified at Cell Signaling Technology using PhosphoScan®, an LC-MS/MS platform used for phosphorylation site discovery (6). Phosphorylation of ALK at Tyr1507 (Tyr567 in NPM-ALK) has been shown to be important for interaction with the adaptor proteins Shc, FRS2-α, and FRS2-β (9,10).
- Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9.
- Iwahara, T. et al. (1997) Oncogene 14, 439-49.
- Morris, S.W. et al. (1997) Oncogene 14, 2175-88.
- Morris, S.W. et al. (1994) Science 263, 1281-4.
- Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
- Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24.
- Soda, M. et al. (2007) Nature 448, 561-6.
- Turner, S.D. et al. (2007) Cell Signal 19, 740-7.
- Chikamori, M. et al. (2007) Oncogene 26, 2950-4.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 7727 Biotinylated Protein Ladder Detection Pack
For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5 is a registered trademark of Biostatus Limited.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
PhosphoScan is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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