Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Sec24C (D9M4N) Rabbit mAb #14676

No. Size Price
14676S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14676 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 120 Rabbit IgG
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Sec24C (D9M4N) Rabbit mAb recognizes endogenous levels of total Sec24C protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro288 of human Sec24C protein.



Confocal immunofluorescent analysis of A-204 cells using Sec24C (D9M4N) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Sec24C (D9M4N) Rabbit mAb.


Coat Protein Complex II (COPII) is composed of five cytosolic proteins: Sec23/24 complex, Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex, thereby forming a prebudding complex that directly binds target molecules (1-3). The prebudding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec24 subunit of COPII coat is thought to play a critical role in cargo selection (2,6). It binds directly to cargo proteins at the ER and brings them to COPII vesicles through interaction with Sec23. There are four Sec24 isoforms in human cells: Sec24A, Sec24B, Sec24C, and Sec24D (7). In mice, mutations in Sec24B have been linked to developmental defects (8,9).

  1. Aridor, M. et al. (1998) J Cell Biol 141, 61-70.
  2. Miller, E.A. et al. (2003) Cell 114, 497-509.
  3. Mossessova, E. et al. (2003) Cell 114, 483-95.
  4. Barlowe, C. et al. (1994) Cell 77, 895-907.
  5. Bi, X. et al. (2007) Dev Cell 13, 635-45.
  6. Miller, E. et al. (2002) EMBO J 21, 6105-13.
  7. Tang, B.L. et al. (1999) Biochem Biophys Res Commun 258, 679-84.
  8. Merte, J. et al. (2010) Nat Cell Biol 12, 41-6; sup pp 1-8.
  9. Wansleeben, C. et al. (2010) Development 137, 1067-73.

Application References

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