Product Pathways - Metabolism
UCP1 (D9D6X) Rabbit mAb #14670
|14670S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|14670||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Species predicted to react based on 100% sequence homology: Human,
Specificity / Sensitivity
UCP1 (D9D6X) Rabbit mAb recognizes endogenous levels of total UCP1 protein. This antibody does not cross-react with UCP2 and UCP3 proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UCP1 protein.
Western blot analysis of extracts from mouse brown adipose tissue (positive) and mouse prostate (negative) using UCP1 (D9D6X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Uncoupling protein 1 (UCP1) is a mitochondrial inner membrane transport protein that is primarily expressed in brown adipose tissue (BAT). UCP1 dissipates the pH gradient resulting from oxidative phosphorylation, which uncouples ATP synthesis from oxidative phosphorylation and leads to the release of heat energy. As a result, UCP1 plays an important role in thermogenesis (reviewed in 1). Research studies indicate that subcutaneous white adipose depots in mice contain beige adipocytes that express low levels of UCP1 protein (2). Additional studies show possible differences in thermogenesis in individuals carrying specific polymorphisms in the corresponding UCP1 gene (3). Related studies link UCP1 to the possible development of obesity and type 2 diabetes (4).
- Azzu, V. and Brand, M.D. (2010) Trends Biochem Sci 35, 298-307.
- Wu, J. et al. (2012) Cell 150, 366-76.
- Nagai, N. et al. (2003) J Clin Endocrinol Metab 88, 5661-7.
- Brondani, L.A. et al. (2012) Arq Bras Endocrinol Metabol 56, 215-25.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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