Cell Signaling Technology

Product Pathways - Metabolism

UCP1 (D9D6X) Rabbit mAb #14670

sc-6528  

No. Size Price
14670S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14670 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 30 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Human,

Specificity / Sensitivity

UCP1 (D9D6X) Rabbit mAb recognizes endogenous levels of total UCP1 protein. This antibody does not cross-react with UCP2 and UCP3 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UCP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brown adipose tissue (positive) and mouse prostate (negative) using UCP1 (D9D6X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of UCP1 from mouse brown adipose tissue extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or UCP1 (D9D6X) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UCP1 (D9D6X) Rabbit mAb.

Background

Uncoupling protein 1 (UCP1) is a mitochondrial inner membrane transport protein that is primarily expressed in brown adipose tissue (BAT). UCP1 dissipates the pH gradient resulting from oxidative phosphorylation, which uncouples ATP synthesis from oxidative phosphorylation and leads to the release of heat energy. As a result, UCP1 plays an important role in thermogenesis (reviewed in 1). Research studies indicate that subcutaneous white adipose depots in mice contain beige adipocytes that express low levels of UCP1 protein (2). Additional studies show possible differences in thermogenesis in individuals carrying specific polymorphisms in the corresponding UCP1 gene (3). Related studies link UCP1 to the possible development of obesity and type 2 diabetes (4).

  1. Azzu, V. and Brand, M.D. (2010) Trends Biochem Sci 35, 298-307.
  2. Wu, J. et al. (2012) Cell 150, 366-76.
  3. Nagai, N. et al. (2003) J Clin Endocrinol Metab 88, 5661-7.
  4. Brondani, L.A. et al. (2012) Arq Bras Endocrinol Metabol 56, 215-25.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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