Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

PRMT6 (D5A2N) Rabbit mAb #14641

methyltransferase   PRMT   sc-55702  

No. Size Price
14641S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14641 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 42 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Bovine, S. cerevisiae,

Specificity / Sensitivity

PRMT6 (D5A2N) Rabbit mAb recognizes endogenous levels of total PRMT6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala62 of human PRMT6 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PRMT6 (D5A2N) Rabbit mAb.

IP

IP

Immunoprecipitation of PRMT6 from MCF7 extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PRMT6 (D5A2N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PRMT6 (D5A2N) Rabbit mAb.

Background

Protein arginine N-methyltransferase 6 (PRMT6) is a member of the protein arginine N-methyltransferase (PRMT) family of proteins that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (1). The three types of PRMTs share the ability to mono-methylate arginine residues, but vary in their ability to generate differential methylation states (1-3). Mono-methylated arginine residues are further methylated by type I PRMTs to generate an asymmetric di-methyl arginine or by type II PRMTs to form a symmetric-dimethyl arginine. Type III methyltransferases are only able to mono-methylate arginine residues (1-3). PRMT6 is a type I PRMT that acts as both a transcriptional coactivator and a corepressor and catalyzes the asymmetric di-methylation of histone H3 (Arg 2, Arg42), histone H4 (Arg3), and histone H2A at Arg29 (2,4). PRMT6 acts as a coactivator for transcription factors, including estrogen receptor and NFκB, while asymmetric di-methylation of histone H3 (Arg2) by PRMT6 prevents MLL methylation of histone H3 at Lys4 and inhibits transcription activation (5-8). In addition to its role in regulating transcription, PRMT6 methylates DNA polymerase β, leading to enhanced DNA binding and processivity during base excision repair of damaged DNA (9).

  1. Di Lorenzo, A. and Bedford, M.T. (2011) FEBS Lett 585, 2024-31.
  2. Yang, Y. and Bedford, M.T. (2013) Nat Rev Cancer 13, 37-50.
  3. Molina-Serrano, D. et al. (2013) Biochem Soc Trans 41, 751-9.
  4. Casadio, F. et al. (2013) Proc Natl Acad Sci U S A 110, 14894-9.
  5. Harrison, M.J. et al. (2010) Nucleic Acids Res 38, 2201-16.
  6. Di Lorenzo, A. et al. (2014) Nucleic Acids Res 42, 8297-309.
  7. Hyllus, D. et al. (2007) Genes Dev 21, 3369-80.
  8. Smith, A.P. et al. (2009) Oncogene 28, 422-30.
  9. El-Andaloussi, N. et al. (2006) Mol Cell 22, 51-62.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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