Cell Signaling Technology

Product Pathways - Neuroscience

RanBP9 (D8M8D) Rabbit mAb #14638

No. Size Price
14638S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14638 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 80, 91 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

RanBP9 (D8M8D) Rabbit mAb recognizes endogenous levels of total RanBP9 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His490 of human RanBP9 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or RanBP9 siRNA (+), using RanBP9 (D8M8D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C2C12 cells using RanBP9 (D8M8D) Rabbit mAb.

Background

RanBP9 (RanBPM) is a Ran binding protein ubiquitously expressed and highly conserved in different organisms. It is a scaffolding protein that was identified as an interacting protein of MET inducing Ras- Erk activation (1). Several domains and motifs regulate the nuclear and cytoplasmic localization of RanBP9 (2). Research studies indicate that RanBP9 might contribute to the pathogenesis of Alzheimer’s Disease (AD). In addition to elevated expression levels in AD samples, this may involve interaction of RanBP9 with APP and BACE1 promoting endocytosis of APP and increased BACE1 mediated cleavage of APP to generate β-amyloid peptides (3).

  1. Wang, D. et al. (2002) J Biol Chem 277, 36216-22.
  2. Salemi, L.M. et al. (2015) PLoS One 10, e0117655.
  3. Lakshmana, M.K. et al. (2009) J Biol Chem 284, 11863-72.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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