Product Pathways - Cell Cycle / Checkpoint
CLASP2 (D5K3E) Rabbit mAb #14629
|14629S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|14629||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Specificity / Sensitivity
CLASP2 (D5K3E) Rabbit mAb recognizes endogenous levels of total CLASP2 protein. This antibody also cross-reacts with an unidentified protein of 95 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala289 of human CLASP2 protein.
Microtubules (MTs) are polarized cellular filaments composed of α/β tubulin heterodimers. The slower growing (minus) microtubule ends are located at MT organizing centers (MTOCs), with the faster growing (plus) ends extending to the cell periphery. The regulation of MT dynamics is an important part of several biological processes, including cell division, migration, adhesion, membrane trafficking, and polarity (1).
Human cytoplasmic linker-associate proteins 1 and 2 (CLASP1 and CLASP2) are evolutionarily conserved proteins that localize to the plus ends of interphase microtubules. During mitosis, CLASP 1 and CLASP2 localize to the centrosomes and kinetochores (KT) where they regulate mitotic spindle positioning to ensure proper chromosome alignment (2,3). Research studies indicate that phosphorylation of the carboxy terminus of CLASP2 during mitosis by CDK1 and PLK1 is required for efficient mitotic MT-KT attachment (4). Phosphorylation of CLASP2 at Ser1013 is a critical step that primes CLASP2 for further phosphorylation by PLK1 (4). The additional phosphorylation of CLASP2 at Ser533 and Ser537 by GSK3-3β controls the distribution of CLASP2 on MTs by inhibiting CLASP2 interaction with the Rac1/cdc42 effector protein IQGAP1 (5).
- Wiese, C. and Zheng, Y. (2006) J Cell Sci 119, 4143-53.
- Mimori-Kiyosue, Y. et al. (2005) J Cell Biol 168, 141-53.
- Logarinho, E. et al. (2012) Nat Cell Biol 14, 295-303.
- Maia, A.R. et al. (2012) J Cell Biol 199, 285-301.
- Watanabe, T. et al. (2009) J Cell Sci 122, 2969-79.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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