Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

ING1b (D3D5Z) Rabbit mAb #14625

No. Size Price
14625S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
14625 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 33 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

ING1b (D3D5Z) Rabbit mAb recognizes endogenous levels of total ING1b protein. This antibody specifically recognizes the ING1b p33 isoform (UniProt# Q9UK53-2).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ING1b protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ING1b (D3D5Z) Rabbit mAb.



Immunoprecipitation of ING1b from HT-29 extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2) or ING1b (D3D5Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ING1b (D3D5Z) Rabbit mAb.


Inhibitor of growth 1 (ING1) is a member of an evolutionarily conserved family of tumor suppressor proteins and transcription regulators (1,2). Differential mRNA splicing generates several ING1 isoforms, with the widely expressed ING1b (p33) isoform playing key roles in cell cycle regulation, apoptosis, and senescence (2-5). All ING family proteins contain a plant homeodomain (PHD) that is thought to recognize and bind methylated lysine residues on histone proteins (6,7). The ING1 protein regulates expression of genes through its association with histone acetyltransferase and deacetylase complexes. The PHD of ING1 may facilitate the recruitment of these chromatin-modifying enzymes to targets genes that are regulated by various transcription factors, such as p53 (2, 8-10). Consistent with its role as a tumor suppressor, alterations in ING1 expression levels and cytoplasm localization have been observed in several cancers but mutations in the corresponding ING1 gene in cancers are uncommon (11-14).

  1. Unoki, M. et al. (2009) Cancer Sci 100, 1173-9.
  2. Nouman, G.S. et al. (2003) J Clin Pathol 56, 491-6.
  3. Helbing, C.C. et al. (1997) Cancer Res 57, 1255-8.
  4. Shinoura, N. et al. (1999) Cancer Res 59, 5521-8.
  5. Abad, M. et al. (2011) Aging Cell 10, 158-71.
  6. Garkavtsev, I. et al. (1996) Nat Genet 14, 415-20.
  7. He, G.H. et al. (2005) Mol Biol Evol 22, 104-16.
  8. Pedeux, R. et al. (2005) Mol Cell Biol 25, 6639-48.
  9. Kuzmichev, A. et al. (2002) Mol Cell Biol 22, 835-48.
  10. Peña, P.V. et al. (2008) J Mol Biol 380, 303-12.
  11. Toyama, T. et al. (1999) Oncogene 18, 5187-93.
  12. Ohmori, M. et al. (1999) Am J Hematol 62, 118-9.
  13. Tallen, G. et al. (2004) Int J Cancer 109, 476-9.
  14. Li, X.H. et al. (2011) Histol Histopathol 26, 597-607.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :