Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

Endoglin (3A9) Mouse mAb #14606

No. Size Price
14606S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14606 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 95 Mouse IgG1
IHC-P 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Endoglin (3A9) Mouse mAb recognizes endogenous levels of total Endoglin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with purified recombinant fragment of human CD105 expressed in E. Coli.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HUVEC (left) and HeLa (right) cell pellets using Endoglin (3A9) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Endoglin (3A9) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HUVEC cells (+) and A172 cells (-) using Endoglin (3A9) Mouse mAb (upper) or β-Actin (#8457) (lower) as a loading control.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using Endoglin (3A9) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma in situ using Endoglin (3A9) Mouse mAb.

Background

Endoglin (ENG, CD105) is an auxiliary receptor for the TGF-β receptor complex, functioning in related signaling pathways (1,2). Endoglin is a transmembrane protein that exists as a disulfide-linked homodimer. It is mainly expressed in vascular and connective tissues and in endothelial and stromal cells. Upregulated endoglin expression has been reported during wound healing and tumor vascularization, and in inflammatory tissues and developing embryos (1-4). Mutations in endoglin have been found to be a causal factor of hereditary hemorrhagic telangiectasia (HHT), a disease characterized by malformation of vascular structure (5,6). The importance of this protein for normal and tumor vascular function makes it a good marker for endothelial cell proliferation as well as a potential therapeutic target in cancer (4-6).

  1. Bernabeu, C. et al. (2007) J Cell Biochem 102, 1375-88.
  2. Zhu, Y. et al. (2003) Stroke 34, 2483-8.
  3. Quintanilla, M. et al. (2003) Oncogene 22, 5976-85.
  4. Dallas, N.A. et al. (2008) Clin Cancer Res 14, 1931-7.
  5. ten Dijke, P. et al. (2008) Angiogenesis 11, 79-89.
  6. Fernández-L, A. et al. (2006) Clin Med Res 4, 66-78.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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