Cell Signaling Technology

Product Pathways - Transcription Factors

NRF2 (D9J1B) Rat mAb (IF Specific) #14596

No. Size Price
14596S 100 µl ( 400 tests ) ¥3,100.00 现货查询 购买询价
14596 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IF-IC 1:400 Mouse, Endogenous 97-100 Rat IgG2b

Species cross-reactivity is determined by western blot.

Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

NRF2 (D9J1B) Rat mAb (IF Specific) recognizes endogenous levels of total NRF2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Ala200 of mouse NRF2 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MEF NRF2 wild-type cells, untreated (left) or treated with MG-132 #2194 (10 μM, 8 hr; center) and MEF NRF2 knock-out cells treated with MG-132 #2194 (10 μM, 8 hr; right), using NRF2 (D9J1B) Rat mAb (IF Specific) (green pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 Phalloidin #8878 (red pseudocolor).

Background

The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintain cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5).

  1. Cullinan, S.B. et al. (2004) Mol Cell Biol 24, 8477-86.
  2. Nguyen, T. et al. (2005) J Biol Chem 280, 32485-92.
  3. Jaiswal, A.K. (2004) Free Radic Biol Med 36, 1199-207.
  4. Suzuki, M. et al. (2008) Am J Respir Cell Mol Biol 39, 673-82.
  5. Homma, S. et al. (2009) Clin Cancer Res 15, 3423-32.

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