Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Nucleolin (D4C7O) Rabbit mAb #14574

No. Size Price
14574S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14574 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 100 Rabbit IgG
IP 1:50
F 1:400
IF-IC 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Nucleolin (D4C7O) Rabbit mAb recognizes endogenous levels of total nucleolin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly53 of human nucleolin protein.



Confocal immunofluorescent analysis of HeLa cells using Nucleolin (D4C7O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Nucleolin (D4C7O) Rabbit mAb.



Immunoprecipitation of nucleolin from Huh7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Nucleolin (D4C7O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Nucleolin (D4C7O) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using Nucleolin (D4C7O) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


Nucleolin is a multi-functional protein that is one of the major components of the nucleoli (1). Nucleolin plays an essential role in various steps of ribosome biogenesis including rRNA synthesis, processing of pre-rRNA, pre-ribosomal RNA assembly, and transport of ribosomal proteins out of the nucleus (1-3). While the main function of nucleolin is ribosome biogenesis, it plays an important role in various other nuclear activities. Down regulation of nucleolin leads to increased expression of p53, defects in genome duplication, and a delay at prometaphase during mitosis leading to cell cycle arrest (4-6). In addition, nucleolin has been found in a complex with Rad51 and may participate in DNA repair by homologous recombination (7). Nucleolin binds to the catalytic subunit of the human telomerase reverse transcriptase, hTERT, and is thought to be involved in telomere maintenance (8). Nucleolin also possesses histone chaperone activity and is able to enhance the chromatin remodeling efficiency of SWItch/Sucrose Non Fermentable (SWI/SNF) and ATP-dependent chromatin-assembly factor (ACF), remove histone H2A-H2B dimers from nucleosomes, and facilitate the passage of RNA polymerase through chromatin (9).

  1. Tajrishi, M.M. et al. (2011) Commun Integr Biol 4, 267-75.
  2. Ginisty, H. et al. (1999) J Cell Sci 112 ( Pt 6), 761-72.
  3. Srivastava, M. and Pollard, H.B. (1999) FASEB J 13, 1911-22.
  4. Takagi, M. et al. (2005) Cell 123, 49-63.
  5. Ugrinova, I. et al. (2007) BMC Mol Biol 8, 66.
  6. Ma, N. et al. (2007) J Cell Sci 120, 2091-105.
  7. De, A. et al. (2006) Biochem Biophys Res Commun 344, 206-13.
  8. Khurts, S. et al. (2004) J Biol Chem 279, 51508-15.
  9. Angelov, D. et al. (2006) EMBO J 25, 1669-79.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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