Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

B7-H4 (D1M8I) XP® Rabbit mAb #14572

B7-H4   B7H4   B7S1   B7x   VTCN1  

No. Size Price
14572S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
14572T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
14572 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 75 Rabbit IgG
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

B7-H4 (D1M8I) XP® Rabbit mAb recognizes endogenous levels of total B7-H4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala141 of human B7-H4 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded SK-BR-3 (left) and NCI-H1299 (right) cell pellets using B7-H4 (D1M8I) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using B7-H4 (D1M8I) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lobular carcinoma of the breast using B7-H4 (D1M8I) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-BR-3, T-47D, and NCI-H1299 cells using B7-H4 (D1M8I) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human PD-L1, PD-L2, B7-H3, or B7-H4 protein (as indicated), using B7-H4 (D1M8I) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

B7 homolog 4 (B7-H4, VTCN1) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses (1-3). B7-H4 protein contains two extracellular Ig-like V-type domains, a transmembrane domain, and a short, two amino acid intracellular domain (3). The B7-H4 protein is shown to inhibit T cell activation, proliferation, and cytokine production (1,4,5). Although B7-H4 mRNA is widely expressed, B7-H4 protein is restricted to antigen presenting cells and B cells (1). The B7-H4 protein is also found in several tumor types, including ovarian cancer and breast cancer (6). Research studies indicate that B7-H4 protein is present on the surface of ovarian tumor cells, and that targeted inhibition of B7-H4 using recombinant antibodies restores T cell activation pathways. These studies suggest some potential therapeutic value in blocking B7-H4 function and restoring T cell function in cancer patients (7,8).

  1. Sica, G.L. et al. (2003) Immunity 18, 849-61.
  2. Prasad, D.V. et al. (2003) Immunity 18, 863-73.
  3. Zang, X. et al. (2003) Proc Natl Acad Sci U S A 100, 10388-92.
  4. He, C. et al. (2011) Clin Dev Immunol 2011, 695834.
  5. Yi, K.H. and Chen, L. (2009) Immunol Rev 229, 145-51.
  6. Salceda, S. et al. (2005) Exp Cell Res 306, 128-41.
  7. Dangaj, D. and Scholler, N. (2013) Oncoimmunology 2, e25913.
  8. Dangaj, D. et al. (2013) Cancer Res 73, 4820-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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