Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CoREST (D6I2U) Rabbit mAb #14567

No. Size Price
14567S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
14567 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 65 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CoREST (D6I2U) Rabbit mAb recognizes endogenous levels of total CoREST protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CoREST protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from LNCaP, DLD-1, and HT-29 cells using CoREST (D6I2U) Rabbit mAb.


The REST corepressor 1 (CoREST, RCOR1) was first identified as a repressor element 1-silencing transcription factor (REST) corepressor (1,2). The CoREST protein is encoded by the RCOR1 gene and is part of a large, multi-subunit repressor complex that includes the histone demethylase LSD1 and histone deacetylases (HDAC) 1 and 2 (1,3-5). CoREST binds the carboxy-terminal domain of REST and is recruited to repress neuronal gene transcription in non-neuronal and neural stem cells (1,6,7). The REST corepressor is essential for repressor complex-nucleosome interaction, the subsequent deacetylation of histone amino-terminal tails by HDAC1/2, and the LSD1 methylation of histone H3 at Lys4 (8-10). The targeting of CoREST to genes that are not repressed by REST suggests a role apart from neural cell fate regulation. These include growth factor independent (Gfi) target genes during erythroid differentiation, targets of carboxy-terminal binding protein (CtBP), and heat shock and pro-inflammatory response genes (11-15).

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Application References

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