Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CoREST (D6I2U) Rabbit mAb #14567

No. Size Price
14567S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14567 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 65 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CoREST (D6I2U) Rabbit mAb recognizes endogenous levels of total CoREST protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CoREST protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from LNCaP, DLD-1, and HT-29 cells using CoREST (D6I2U) Rabbit mAb.

Background

The REST corepressor 1 (CoREST, RCOR1) was first identified as a repressor element 1-silencing transcription factor (REST) corepressor (1,2). The CoREST protein is encoded by the RCOR1 gene and is part of a large, multi-subunit repressor complex that includes the histone demethylase LSD1 and histone deacetylases (HDAC) 1 and 2 (1,3-5). CoREST binds the carboxy-terminal domain of REST and is recruited to repress neuronal gene transcription in non-neuronal and neural stem cells (1,6,7). The REST corepressor is essential for repressor complex-nucleosome interaction, the subsequent deacetylation of histone amino-terminal tails by HDAC1/2, and the LSD1 methylation of histone H3 at Lys4 (8-10). The targeting of CoREST to genes that are not repressed by REST suggests a role apart from neural cell fate regulation. These include growth factor independent (Gfi) target genes during erythroid differentiation, targets of carboxy-terminal binding protein (CtBP), and heat shock and pro-inflammatory response genes (11-15).

  1. Qureshi, I.A. et al. (2010) Cell Cycle 9, 4477-86.
  2. Barrios, Á.P. et al. (2014) Mol Cell Biol 34, 2760-70.
  3. Andrés, M.E. et al. (1999) Proc Natl Acad Sci U S A 96, 9873-8.
  4. Humphrey, G.W. et al. (2001) J Biol Chem 276, 6817-24.
  5. You, A. et al. (2001) Proc Natl Acad Sci U S A 98, 1454-8.
  6. Ballas, N. et al. (2005) Cell 121, 645-57.
  7. Ballas, N. and Mandel, G. (2005) Curr Opin Neurobiol 15, 500-6.
  8. Lee, M.G. et al. (2005) Nature 437, 432-5.
  9. Shi, Y.J. et al. (2005) Mol Cell 19, 857-64.
  10. Yang, M. et al. (2006) Mol Cell 23, 377-87.
  11. Saleque, S. et al. (2007) Mol Cell 27, 562-72.
  12. Yao, H. et al. (2014) Blood 123, 3175-84.
  13. Gómez, A.V. et al. (2008) Mol Cell 31, 222-31.
  14. Saijo, K. et al. (2009) Cell 137, 47-59.
  15. Chinnadurai, G. (2002) Mol Cell 9, 213-24.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Tween is a registered trademark of ICI Americas, Inc.

CST is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications