Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-DDR1 (Tyr513) (E1N8F) Rabbit mAb #14531

No. Size Price
14531S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
14531 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 125 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-DDR1 (Tyr513) (E1N8F) Rabbit mAb recognizes endogenous levels of DDR1 protein only when phosphorylated at Tyr513.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr513 of human DDR1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from T47-D cells, untreated (-) or treated with collagen (20 μg/ml, 18 hr; +), using Phospho-DDR1 (Tyr513) (E1N8F) Rabbit mAb (upper) and DDR1 (D1G6) XP® Rabbit mAb #5583 (lower).


The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Research studies have implicated aberrant expression and signaling of DDRs in human diseases related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion (1).

Phosphorylation of DDR1 on Tyr513 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST™ LC-MS/MS platform for phosphorylation site discovery (3). Additional research looking at DDR1 activation state has identified the same phosphorylation site in DDR1 (4).

  1. Vogel, W. (1999) FASEB J 13 Suppl, S77-82.
  2. Ferri, N. et al. (2004) Am J Pathol 164, 1575-85.
  3. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
  4. Fu, H.L. et al. (2014) J Biol Chem 289, 9275-87.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

PhosphoScan is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

CST is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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