Cell Signaling Technology

Product Pathways - Protein Stability

UBQLN1 (D3T7F) Rabbit mAb #14526

hPLIC-1   hPLIC1   Ubiquitin Receptors   UBQLN-1   UBQLN1   Ubquitin Binding Proteins  

No. Size Price
14526S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14526 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 66 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

UBQLN1 (D3T7F) Rabbit mAb recognizes endogenous levels of total UBQLN1 protein. This antibody does not cross-react with other UBQLN proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBQLN1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBQLN1 (D3T7F) Rabbit mAb.

IP

IP

Immunoprecipitation of UBQLN1 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or UBQLN1 (D3T7F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBQLN1 (D3T7F) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UBQLN1 protein (hUBQLN1-Myc/DDK; +), using UBQLN1 (D3T7F) Rabbit mAb.

Background

Ubiquilin 1 (UBQLN1) is a ubiquitously expressed, type 2 ubiquitin like (UBL) protein that contains an amino-terminal UBL domain and a carboxy-terminal Ub-associated (UBA) domain (1). Research studies demonstrate that UBQLN1 associates with poly-Ub chains through its UBA domain, while the UBL domain participates in interactions with proteasome subunits. Evidence suggests that UBQLN1 acts as a shuttling factor during endoplasmic-reticulum-associated protein degradation (ERAD) as it transports misfolded, ubiquitinated proteins from the ER to the proteasome for subsequent degradation (2-5). Additional research studies demonstrate that the UBL domain of UBQLN1 binds UIM-containing endocytic proteins and participates in the sequestration of protein aggregates during aggresome formation (6,7). UBQLN1 regulates presenilin protein levels and is localized in neurofibrillary tangles of Alzheimer's disease-affected brains (8). Polymorphisms in the corresponding UBQLN1 gene may be associated with a risk of Alzheimer's disease (9-11).

  1. Hanaoka, E. et al. (2000) J Hum Genet 45, 188-91.
  2. Ko, H.S. et al. (2004) FEBS Lett 566, 110-4.
  3. Lim, P.J. et al. (2009) J Cell Biol 187, 201-17.
  4. Kleijnen, M.F. et al. (2000) Mol Cell 6, 409-19.
  5. Zhang, D. et al. (2008) J Mol Biol 377, 162-80.
  6. Heir, R. et al. (2006) EMBO Rep 7, 1252-8.
  7. Regan-Klapisz, E. et al. (2005) J Cell Sci 118, 4437-50.
  8. Mah, A.L. et al. (2000) J Cell Biol 151, 847-62.
  9. Yue, Z. et al. (2014) Int J Neurosci [Epub ahead of print ].
  10. Viswanathan, J. et al. (2011) Traffic 12, 330-48.
  11. Bertram, L. et al. (2005) N Engl J Med 352, 884-94.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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