Product Pathways - Neuroscience
EAAT3 (E1E6M) Rabbit mAb #14501
|14501S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|14501||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen),
Specificity / Sensitivity
EAAT3 (E1E6M) Rabbit mAb recognizes endogenous levels of total EAAT3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val510 of human EAAT3 protein.
Confocal immunofluorescent analysis of mouse dentate gyrus using EAAT3 (E1E6M) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from various tissues using EAAT3 (E1E6M) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
During neurotransmission, glutamate is released from vesicles of the presynaptic cell, and glutamate receptors (e.g., NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing postsynaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels (1,2). In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion (1,2). Five EAATs (EAAT1-5) have been identified. EAAT1 and EAAT2 are expressed mainly in glia, while EAAT3, EAAT4, and EAAT5 are considered to be neuronal transporters (2). EAAT3 is found in the perisynaptic areas and cell bodies of glutamatergic and GABAergic neurons (3). Research studies have implicated abnormal EAAT3 expression in the pathophysiology of Schizophrenia (4,5).
- Danbolt, N.C. (2001) Prog Neurobiol 65, 1-105.
- Amara, S.G. and Fontana, A.C. (2002) Neurochem Int 41, 313-8.
- Rothstein, J.D. et al. (1994) Neuron 13, 713-25.
- Bauer, D. et al. (2008) Schizophr Res 104, 108-20.
- Horiuchi, Y. et al. (2012) Am J Med Genet B Neuropsychiatr Genet 159B, 30-7.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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