Cell Signaling Technology

Product Pathways - Innate Immunity

OAS1 (D1W3A) Rabbit mAb #14498

No. Size Price
14498S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14498 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 40, 44 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

OAS1 (D1W3A) Rabbit mAb recognizes endogenous levels of total OAS1 protein. This antibody cross-reacts with an unidentified protein of 100 kDa in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp90 of human OAS1 protien.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NCI-H460, and HT-29 cells, untreated (-) or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml, 16 hr; +), using OAS1 (D1W3A) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human OAS1 isoform p41 (hOAS1 p41; +), using OAS1 (D1W3A) Rabbit mAb.

Background

2’-5’-oligoadenylate synthetase 1 (OAS1) is an antiviral protein induced by type 1 interferon that plays a key role in the cellular innate immune response (1). The OAS family of proteins includes OAS1, OAS2, OAS3, and OASL in humans (2). The OAS1 enzyme produces the second messenger 2’-5’-linked oligoadenylate in response to cytosolic dsRNA. These 2’-5’-linked oligoadenylates bind to the ribonuclease RNase L, which then degrades viral and cellular RNA (3). Research studies indicate that the OAS1 system inhibits protein synthesis and induces apoptosis in virally infected cells, which limits viral infection (4). Alternative splicing generates multiple isoforms of human OAS1, including p41 and the canonical p46 (5,6). Polymorphisms in the corresponding OAS1 gene have been examined for possible association with increased susceptibility to type 1 diabetes mellitus, multiple sclerosis, and infection by viral pathogens (7,8).

  1. Schoggins, J.W. et al. (2011) Nature 472, 481-5.
  2. Chebath, J. et al. (1987) Nature 330, 587-8.
  3. Dong, B. and Silverman, R.H. (1997) J Biol Chem 272, 22236-42.
  4. Castelli, J.C. et al. (1998) Cell Death Differ 5, 313-20.
  5. Benech, P. et al. (1985) EMBO J 4, 2249-56.
  6. Saunders, M.E. et al. (1985) EMBO J 4, 1761-8.
  7. Cagliani, R. et al. (2012) Hum Genet 131, 87-97.
  8. Tessier, M.C. et al. (2006) J Med Genet 43, 129-32.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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