Product Pathways - DNA Damage
RAP80 (D1T6Q) Rabbit mAb #14466
|14466S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|14466||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
RAP80 (D1T6Q) Rabbit mAb recognizes endogenous levels of total RAP80 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly321 of human RAP80 protein.
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® RAP80 siRNA #14519 (+), using RAP80 (D1T6Q) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Western blot analysis of extracts from various cell lines using RAP80 (D1T6Q) Rabbit mAb.
Confocal immunofluorescent analysis of 293T cells either untreated (left) or neocarzinostatin-treated (5 μg/mL; 1hr, right) using RAP80 (D1T6Q) Rabbit mAb (green). RAP80 foci become more numerous with neocarzinostatin treatment. Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red).
The breast cancer type 1 susceptibility protein (BRCA1) is an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of the DNA damage response and DNA repair. BRCA1 protein forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for regulation of BRCA1 protein function. The BRCA1-RAP80 complex (BRCA1 A complex) includes RAP80, BRCC36, BRE, Abraxas, and NBA1 and functions in G2/M phase checkpoint control (reviewed in 1,2).
The ubiquitously expressed receptor-associated protein 80 (RAP80, UIMC1) is required for recruitment and stability of the BRCA1 A complex at sites of DNA damage (3). Research studies indicate that the absence of RAP80 in cells results in increased sensitivity to the topoisomerase II inhibitor etoposide (4). In the absence of functional RAP80, BRCA1 A complex function is suppressed and cells become more sensitive to DNA damage-induced genome instability (5,6). Phosphorylation of RAP80 by CDK1/Cyclin B at Ser177 regulates RAP80 function at the mitotic checkpoint (7). A naturally occurring in-frame deletion mutant within RAP80 likely alters RAP80 protein-protein interactions and is associated with an increase in chromosomal abnormalities (8,9).
- Ohta, T. et al. (2011) FEBS Lett 585, 2836-44.
- Huen, M.S. et al. (2010) Nat Rev Mol Cell Biol 11, 138-48.
- Wu, J. et al. (2012) J Biol Chem 287, 22919-26.
- Iijima, J. et al. (2010) Cancer Res 70, 8467-74.
- Bian, C. et al. (2012) PLoS One 7, e40406.
- Yin, Z. et al. (2012) Cancer Res 72, 5080-90.
- Cho, H.J. et al. (2013) J Biol Chem 288, 3768-76.
- Nikkilä, J. et al. (2009) Oncogene 28, 1843-52.
- Anamika et al. (2014) J Biol Chem 289, 12852-62.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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