Cell Signaling Technology

Product Pathways - Screening Technologies

Phospho-Ser-Pro-Pro Motif [pSPP] Rabbit mAb #14390

Motif   SPP   substrate  

No. Size Price
14390S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
14390 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Ser-Pro-Pro Motif [pSPP] Rabbit mAb recognizes endogenous levels of proteins only when phosphorylated at serine within the context of the SPP motif. This antibody does not cross-react with proteins phosphorylated at threonine, or with serine residues phosphorylated in other contexts.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing the phospho-Ser-Pro-Pro motif.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116 cells, G0 cells that were serum starved and contact inhibited (36 hr) or mitotic cells treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (50 ng/ml, 20 hr), using Phospho-Ser-Pro-Pro Motif [pSPP] Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To facilitate the study and discovery of new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine or phospho-serine followed by proline.

The S/TPP motif matches the consensus sequence for targeted phosphorylation by Cdc2 (7,8), ERK1, ERK2 (9), SAPK/JNK, CDK5, and GSK3 (10).

  1. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  2. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
  3. Nurse, P. (2000) Cell 100, 71-8.
  4. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
  5. Yang, C.C. et al. (1998) J Protein Chem 17, 329-35.
  6. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
  7. Shah, O.J. et al. (2003) J Biol Chem 278, 16433-42.
  8. Songyang, Z. et al. (1994) Curr Biol 4, 973-82.
  9. Gonzalez, F.A. et al. (1991) J Biol Chem 266, 22159-63.
  10. Yang, X. and Gabuzda, D. (1998) J Biol Chem 273, 29879-87.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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