Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MAPK Substrates Motif [PXpTP] MultiMab™ Rabbit mAb mix #14378


No. Size Price
14378S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
14378 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MAPK Substrates Motif [PXpTP] MultiMab™ Rabbit mAb mix recognizes endogenous levels of proteins that are phosphorylated at threonine within the context of a PXTP motif. The antibody does not cross-react with endogenous levels of non-phosphorylated proteins, phospho-threonine in another context, or proteins with a phosphorylated serine within the PXSP motif.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing the phospho-PXTP motif.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116 cells, G0 cells that were serum starved and contact inhibited (36 hr), or mitotic cells treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (50 ng/ml, 20 hr), using Phospho-MAPK Substrates Motif [PXpTP] MultiMab™ Rabbit mAb mix (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).


The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K. Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*/T*P and/or S*PXR/K, for use in the study and discovery of new MAPK and CDK substrates (4,5).

  1. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
  2. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
  3. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
  4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
  5. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

MultiMab is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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