Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Gα(q) (D5V1B) Rabbit mAb #14373

sc-393  

No. Size Price
14373S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14373 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 40 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Gα(q) (D5V1B) Rabbit mAb recognizes endogenous levels of total Gα(q) protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn126 of human Gα(q) protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and RL-7 cells using Gα(q) (D5V1B) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines, and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).

The Gα(q) guanine nucleotide-binding protein mediates communication between cell surface receptors and the key signal transduction enzyme phospholipase C-β (4). Mutations in the corresponding GNAQ gene can result in Sturge-Weber syndrome, a neurological and skin disorder characterized by facial port-wine stains, glaucoma, seizures, and mental retardation (5).

  1. Offermanns, S. (2001) Oncogene 20, 1635-42.
  2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
  3. Umemori, H. et al. (1997) Science 276, 1878-81.
  4. Hubbard, K.B. and Hepler, J.R. (2006) Cell Signal 18, 135-50.
  5. Shirley, M.D. et al. (2013) N Engl J Med 368, 1971-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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