Product Pathways - Cell Cycle / Checkpoint
Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb #14371
|14371S||100 µl ( 10 western blots )||￥4,050.00||现货查询 购买询价 防伪查询|
|14371||carrier free & custom formulation / quantity||email request|
|W||1:1000||Human,All Species Expected,||Endogenous||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb recognizes endogenous levels of proteins that are phosphorylated at threonine within the context of a TPXK motif. The antibody does not cross-react with non-phosphorylated proteins or phospho-threonine in a different context.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing the phospho-TPXK motif.
Western blot analysis of extracts from HCT 116 cells, G0 cells that were serum starved and contact inhibited (36 hr) or mitotic cells treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (50 ng/ml, 20 hr), using Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunoprecipitation of phospho-TPXK motif-containing proteins from mitotic HCT 116 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 or Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb. Lane 1 is 10% input. Western blot analysis was performed using Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb followed by Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 as secondary antibodies.
Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transition through their association and subsequent phosphorylation of substrate proteins in a proline-directed fashion. CDKs phosphorylate a serine or threonine residue within the (S/T)PX(R/K) consensus sequence (1-5). Cell Signaling Technology has developed antibodies to phosphorylated proline-rich motifs for broad-based discovery applications. The TPXK motif is targeted for phosphorylation by CDKs 1, 2, 4, 5, and 6 (1-5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at firstname.lastname@example.org.
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