Product Pathways - Chromatin Regulation / Epigenetics
MED12 (D9K5J) Rabbit mAb #14360
|14360S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|14360||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
MED12 (D9K5J) Rabbit mAb recognizes endogenous levels of total MED12 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1813 of human MED12 protein.
Western blot analysis of extracts from various cell lines using MED12 (D9K5J) Rabbit mAb.
The mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors (1). Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment (1-5). The mediator complex also plays an important role in creating ‘chromatin loops’ that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter, and works to sustain proper chromatin architecture during active transcription (6-8).
MED12 is part of the CDK8 submodule of the mediator complex and is required for the stable interaction of this module with the rest of the mediator complex (1,9). The CDK8 module has been shown to be both a negative and positive regulator of transcription, depending on the gene context. The CDK8 module may repress transcription by inhibiting the ability of mediator to recruit RNAPII (10). In addition, the MED12 subunit can recruit the methyltransferase G9a to methylate histone H3K9 to repress a subset of neuronal genes in non-neuronal cells (11). MED12 and the CDK8 module can also positively regulate transcription of Wnt-responsive genes through its interaction with β-catenin, and p53-regulated genes upon UV-induced DNA damage (1,9,12).
- Taatjes, D.J. (2010) Trends Biochem Sci 35, 315-22.
- Kornberg, R.D. (2005) Trends Biochem Sci 30, 235-9.
- Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63.
- Malik, S. et al. (2002) Mol Cell Biol 22, 5626-37.
- Wang, G. et al. (2005) Mol Cell 17, 683-94.
- Kagey, M.H. et al. (2010) Nature 467, 430-5.
- Park, S.W. et al. (2005) Mol Cell 19, 643-53.
- Chen, Z. et al. (2011) EMBO J 30, 2405-19.
- Kim, S. et al. (2006) J Biol Chem 281, 14066-75.
- Knuesel, M.T. et al. (2009) Genes Dev 23, 439-51.
- Ding, N. et al. (2008) Mol Cell 31, 347-59.
- Galbraith, M.D. et al. (2010) Transcription 1, 4-12.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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