Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MED12 (D9K5J) Rabbit mAb #14360

No. Size Price
14360S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14360 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 240 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MED12 (D9K5J) Rabbit mAb recognizes endogenous levels of total MED12 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1813 of human MED12 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MED12 (D9K5J) Rabbit mAb.

IP

IP

Immunoprecipitation of MED12 from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MED12 (D9K5J) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MED12 (D9K5J) Rabbit mAb.

Background

The mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors (1). Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment (1-5). The mediator complex also plays an important role in creating ‘chromatin loops’ that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter, and works to sustain proper chromatin architecture during active transcription (6-8).

MED12 is part of the CDK8 submodule of the mediator complex and is required for the stable interaction of this module with the rest of the mediator complex (1,9). The CDK8 module has been shown to be both a negative and positive regulator of transcription, depending on the gene context. The CDK8 module may repress transcription by inhibiting the ability of mediator to recruit RNAPII (10). In addition, the MED12 subunit can recruit the methyltransferase G9a to methylate histone H3K9 to repress a subset of neuronal genes in non-neuronal cells (11). MED12 and the CDK8 module can also positively regulate transcription of Wnt-responsive genes through its interaction with β-catenin, and p53-regulated genes upon UV-induced DNA damage (1,9,12).

  1. Taatjes, D.J. (2010) Trends Biochem Sci 35, 315-22.
  2. Kornberg, R.D. (2005) Trends Biochem Sci 30, 235-9.
  3. Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63.
  4. Malik, S. et al. (2002) Mol Cell Biol 22, 5626-37.
  5. Wang, G. et al. (2005) Mol Cell 17, 683-94.
  6. Kagey, M.H. et al. (2010) Nature 467, 430-5.
  7. Park, S.W. et al. (2005) Mol Cell 19, 643-53.
  8. Chen, Z. et al. (2011) EMBO J 30, 2405-19.
  9. Kim, S. et al. (2006) J Biol Chem 281, 14066-75.
  10. Knuesel, M.T. et al. (2009) Genes Dev 23, 439-51.
  11. Ding, N. et al. (2008) Mol Cell 31, 347-59.
  12. Galbraith, M.D. et al. (2010) Transcription 1, 4-12.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications