Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

NFAT1 (D43B1) XP® Rabbit mAb (PE Conjugate) #14335

No. Size Price
14335S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
14335 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

NFAT1 (D43B1) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of total NFAT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly87 of human NFAT1 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated NFAT1 (D43B1) XP® Rabbit mAb #5861.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of LNCaP (blue) and Jurkat (green) cells using NFAT1 (D43B1) XP® Rabbit mAb (PE Conjugate).

Background

The NFAT (nuclear factor of activated T cells) family of proteins consists of NFAT1 (NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response (1,2). NFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation programs in these cells (3). In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus (2). Termination of NFAT signaling occurs upon declining calcium concentrations and phosphorylation of NFAT by kinases such as GSK-3 or CK1 (3,4). Cyclosporin A and FK506 are immunosuppressive drugs that inhibit calcineurin and thus retain NFAT proteins in the cytoplasm (5).

  1. Northrop, J.P. et al. (1993) J Biol Chem 268, 2917-23.
  2. Hogan, P.G. et al. (2003) Genes Dev 17, 2205-32.
  3. Crabtree, G.R. and Olson, E.N. (2002) Cell 109 Suppl, S67-79.
  4. Okamura, H. et al. (2004) Mol Cell Biol 24, 4184-95.
  5. Shaw, K.T. et al. (1995) Proc Natl Acad Sci U S A 92, 11205-9.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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