Cell Signaling Technology

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Hematoxylin #14166

blue   counterstain   Gill   Gill''s   hema   histo   ihc   immunohistochemistry   nuclear   signal stain   signalstain   stain  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IHC-P
IHC-F

Species cross-reactivity is determined by western blot.

Applications Key: IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen),

Description

Hematoxylin is a blue nuclear counterstain for use in immunohistochemical assays. It yields crisp staining detail with superior contrast when used in conjunction with SignalStain® DAB Substrate Kit #8059. It is also compatible with SignalStain® Mounting Medium #14177.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Claudin-1 (D5H1D) XP® Rabbit mAb #13255 (brown) followed by a counterstain with Hematoxylin (blue).

Structure

Structure

Chemical structure of Hematoxylin.

Directions for Use

Hematoxylin is a blue nuclear counterstain for use after the completion of immunohistochemical assays. It yields crisp nuclear staining with superior contrast with SignalStain® DAB Substrate Kit #8059 and is also compatible with SignalStain® Mounting Medium #14177.

A) Solutions and Reagents

Supplied Reagents

1. Hematoxylin #14166

Additional Reagents (Not Supplied)

1. Acid Rinse Solution: To prepare 100ml, add 2ml of glacial acetic acid to 98 ml of dH20.

2. Bluing Solution: To prepare 100ml, add 1.5 ml of 30% ammonium hydroxide (NH4OH) to 98.5ml of 70% ethanol

3. SignalStain® Mounting Medium #14177

B) Nuclear Counterstaining

1. Rinse slides in tap water.

2. Completely submerse slides in Hematoxylin #14166 and incubate for 1-5 minutes.

3. Wash slides with tap water until rinse water is clear.

4. Immerse slides 10 times in acid rinse solution.

5. Rinse slides 10 times in tap water.

6. Immerse slides for 1 minute in bluing solution

7. Rinse slides 10 times in tap water.

8. Mount sections with SignalStain® Mounting Medium #14177.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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