Cell Signaling Technology

Product Pathways - Adhesion

MUC1 (D9O8K) XP® Rabbit mAb #14161

breast carcinoma-associated antigen DF3   CD227 antigen   DF3   episialin   MUC1   mucin-1   Mucin-1. cell surface assciated {MUC1}   mucin1   polymorphic epithelial mucin (PEM)  

No. Size Price
14161S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
14161T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
14161 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 230, 400 Rabbit IgG
IP 1:50
F 1:200
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

MUC1 (D9O8K) XP® Rabbit mAb detects endogenous levels of total MUC1 protein. Because this protein is heavily glycosylated, multiple bands or a smear may result from western blot analysis using this antibody.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MUC1 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of ZR-75-1 (positive, left) and HCT 116 (negative, right) cells using MUC1 (D9O8K) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HCT 116 (blue) and ZR-75-1 (green) using MUC1 (D9O8K) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from ZR-75-1, MCF 10A, and HCT 116 cells using MUC1 (D9O8K) XP® Rabbit mAb (upper) and β-Tubulin Antibody #2146 (lower).

Background

Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a cytoplasmic tail of 72 residues (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ and Src through phosphorylation at Ser44, Thr41 and Tyr46 of MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6), and attenuates stress-induced apoptosis through the Akt or p53 pathways (7,8).

  1. Baldus, S.E. et al. (2004) Crit Rev Clin Lab Sci 41, 189-231.
  2. Schroeder, J.A. et al. (2001) J Biol Chem 276, 13057-64.
  3. Li, Y. et al. (1998) Mol Cell Biol 18, 7216-24.
  4. Li, Y. et al. (2001) J Biol Chem 276, 6061-4.
  5. Ren, J. et al. (2002) J Biol Chem 277, 17616-22.
  6. Schroeder, J.A. et al. (2004) Oncogene 23, 5739-47.
  7. Raina, D. et al. (2004) J Biol Chem 279, 20607-12.
  8. Wei, X. et al. (2005) Cancer Cell 7, 167-78.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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