Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb #14053

No. Size Price
14053S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
14053 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,Rat, Endogenous 45 Rabbit IgG
IF-F 1:200
IF-IC 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Human,

Specificity / Sensitivity

Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb recognizes endogenous levels of doublecortin protein only when phosphorylated at Ser334. Amino acid Ser334 of mouse doublecortin corresponds to Ser415 of human doublecortin. Proteins of unknown origin may be detected around 90 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser334 of mouse doublecortin protein.

IF-F

IF-F

Confocal immunofluorescent analysis of postnatal day 5 rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of P19 differentiated cells using Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after protein transfer.

Background

Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).

Doublecortin phosphorylated at Ser334 is enriched in growth cones and affects neurite outgrowth and neuronal migration (3).

  1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
  2. Reiner, O. et al. (2004) Cell Cycle 3, 747-51.
  3. Gdalyahu, A. et al. (2004) EMBO J 23, 823-32.
  4. Schaar, B.T. et al. (2004) Neuron 41, 203-13.
  5. Tanaka, T. et al. (2004) Neuron 41, 215-27.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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