Cell Signaling Technology

Product Pathways - Protein Stability

Ubiquitin (P4D1) Mouse mAb (HRP Conjugate) #14049

No. Size Price
14049S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
14049 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Ubiquitin (P4D1) Mouse mAb (HRP Conjugate) detects ubiquitin, polyubiquitin, and ubiquitinated proteins. This antibody may cross-react with recombinant NEDD8.

Source / Purification

Monoclonal antibody is produced by immunizing animals with 1-76 full-length bovine ubiquitin.


This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ubiquitin (P4D1) Mouse mAb #3936.

Western Blotting

Western Blotting

Western blot analysis of HeLa cells, untreated (-) or treated with the 26S proteasome inhibitor MG-132 #2194 (50 µM, 90 min; +), using Ubiquitin (P4D1) Mouse mAb (HRP Conjugate).


Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).

  1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
  2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
  3. Hochstrasser, M. (2000) Science 289, 563-4.
  4. Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
  5. Aberle, H. et al. (1997) EMBO J 16, 3797-804.
  6. Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
  7. Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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