Product Pathways - Neuroscience
BMAL1 (D2L7G) Rabbit mAb #14020
|14020S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|14020||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,
Specificity / Sensitivity
BMAL1 (D2L7G) Rabbit mAb recognizes endogenous levels of total BMAL1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly552 of human BMAL1 protein.
Western blot analysis of extracts from various cell lines and tissues using BMAL1 (D2L7G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of BMAL1 from SH-SY5Y cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or BMAL1 (D2L7G) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using BMAL1 (D2L7G) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human BMAL1 protein (hBMAL1-Myc; +), using BMAL1 (D2L7G) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 3T3 cells treated with dexamathasone (1 μM) for 2 hours, and either 10 μl of BMAL1 (D2L7G) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse DBP intron 2 primers, SimpleChIP® Mouse PER1 Intron 1 Primers #13902, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Circadian rhythms govern many key physiological processes that fluctuate with a period of approximately 24 hours. These processes include the sleep-wake cycle, glucose, lipid and drug metabolism, heart rate, hormone secretion, renal blood flow, and body temperature, as well as basic cellular processes such as DNA repair and the timing of the cell division cycle (1,2). The mammalian circadian system consists of many individual tissue-specific clocks (peripheral clocks) that are controlled by a master circadian pacemaker residing in the suprachiasmatic nuclei (SCN) of the brain (1,2). The periodic circadian rhythm is prominently manifested by the light-dark cycle, which is sensed by the visual system and processed by the SCN. The SCN processes the light-dark information and synchronizes peripheral clocks through neural and humoral output signals (1,2).
The cellular circadian clockwork consists of interwoven positive and negative regulatory loops, or limbs (1,2). The positive limb includes the CLOCK and BMAL1 proteins, two basic helix-loop-helix-PAS containing transcription factors that bind E box enhancer elements and activate transcription of their target genes. CLOCK is a histone acetyltransferase (HAT) protein, which acetylates both histone H3 and H4 (3). BMAL1 binds to CLOCK and enhances its HAT activity (3). The CLOCK/BMAL1 dimer exhibits a periodic oscillation in both nuclear/cytoplasmic localization and protein levels, both of which are regulated by phosphorylation (4,5). CLOCK/BMAL1 target genes include the Cry and Per genes, whose proteins form the negative limb of the circadian clockwork system (1,2). CRY and PER proteins (CRY1, CRY2, PER1, PER2 and PER3) form oligomers that also periodically shuttle between the nucleus and cytoplasm. When in the nucleus, CRY/PER proteins inhibit CLOCK/BMAL1-mediated transcriptional activation, thus completing the circadian transcriptional loop (1,2). In tissues, roughly six to eight percent of all genes exhibit a circadian expression pattern (1,2). This 24-hour periodicity in gene expression results from coordination of the positive and negative regulatory limbs of the cellular clockwork system, and is fine-tuned by outside signals received from the SCN.
- Albrecht, U. and Eichele, G. (2003) Curr Opin Genet Dev 13, 271-7.
- Virshup, D.M. et al. (2007) Cold Spring Harb Symp Quant Biol 72, 413-20.
- Doi, M. et al. (2006) Cell 125, 497-508.
- Kondratov, R.V. et al. (2003) Genes Dev 17, 1921-32.
- Kwon, I. et al. (2006) Mol Cell Biol 26, 7318-30.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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