Product Pathways - Metabolism
Miro2 Antibody #14016
|14016S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|14016||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
Miro2 Antibody recognizes endogenous levels of total Miro2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly452 of human Miro2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Miro2 protein (hMiro2-Myc/DDK; +), using Miro2 Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mitochondrial Rho GTPase 1 (Miro1, RHOT1) and mitochondrial Rho GTPase 2 (Miro2, RHOT2) are atypical Ras GTPase proteins that localize to the outer mitochondrial membrane (1,2). These evolutionarily conserved proteins contain GTP-binding domains and a pair of calcium-binding EF hand domains (1,2). Research studies indicate that Miro1 and Miro2 function in the axonal transport of mitochondria in neurons (2). Both Miro proteins play an essential role in mitochondrial trafficking by attaching mitochondria to essential motor and adaptor proteins (3). Miro GTPase proteins that are anchored to the outer mitochondrial membrane interact with kinesin-binding proteins TRAK1 and TRAK2 to provide a link between mitochondria to microtubules (4). Increased levels of synaptic calcium appears to inhibit mitochondrial trafficking mediated by Miro, suggesting a role for the EF hand as a calcium sensor (5).
- Fransson, A. et al. (2003) J Biol Chem 278, 6495-502.
- Anvret, A. et al. (2012) Open Neurol J 6, 1-5.
- Fransson, S. et al. (2006) Biochem Biophys Res Commun 344, 500-10.
- Saotome, M. et al. (2008) Proc Natl Acad Sci U S A 105, 20728-33.
- Macaskill, A.F. et al. (2009) Neuron 61, 541-55.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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