Cell Signaling Technology

Product Pathways - Neuroscience

Na Channel β2 Subunit (D6L5Y) Rabbit mAb #13966

No. Size Price
13966S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13966 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,Rat, Endogenous 37-41 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Na channel β2 subunit (D6L5Y) Rabbit mAb recognizes endogenous levels of total Na channel β2 subunit protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu90 of mouse Na channel β2 subunit protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain membrane using Na Channel β2 Subunit (D6L5Y) Rabbit mAb.

IP

IP

Immunoprecipitation of Na channel β2 subunit from mouse brain extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Na Channel β2 Subunit (D6L5Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Na Channel β2 Subunit (D6L5Y) Rabbit mAb.

Background

Mammalian voltage-gated sodium channels (VGSCs) are composed of a pore-forming α subunit and one or more regulatory β subunits (1). Four separate genes (SCN1B-SCN4B) encode the five mammalian β subunits β1, β1B, β2, β3, and β4. In general, β subunit proteins are type I transmembrane proteins, with the exception of secreted β1B protein (reviewed in 2). β subunits regulate α subunit gating and kinetics, which controls cell excitability (3,4). Sodium channel β subunits also function as Ig superfamily cell adhesion molecules that regulate cell adhesion and migration (5,6). Additional research reveals sequential processing of β subunit proteins by β-secretase (BACE1) and γ secretase, resulting in ectodomain shedding of β subunit and generation of an intracellular carboxy-terminal fragment (CTF). Generation of the CTF is thought to play a role in cell adhesion and migration (7,8). Multiple studies demonstrate a link between β subunit gene mutations and a number of disorders, including epilepsy, cardiac arrhythmia, multiple sclerosis, neuropsychiatric disorders, neuropathy, inflammatory pain, and cancer (9-13).

The sodium channel β2 subunit (SCN2B) plays a role in the assembly and functioning of cell surface sodium channels (14). Mutations in the corresponding SCN2B gene may be associated with a form of atrial fibrillation and at least one identified case of Brugada syndrome (15,16). Research using a rat model of spared nerve injury (SNI) suggests that the β2 subunit may be involved in the generation of neuropathic pain (17).

  1. Catterall, W.A. (1992) Physiol Rev 72, S15-48.
  2. Catterall, W.A. (2012) J Physiol 590, 2577-89.
  3. Isom, L.L. et al. (1992) Science 256, 839-42.
  4. Brackenbury, W.J. and Isom, L.L. (2011) Front Pharmacol 2, 53.
  5. Isom, L.L. et al. (1995) Cell 83, 433-42.
  6. Malhotra, J.D. et al. (2000) J Biol Chem 275, 11383-8.
  7. Wong, H.K. et al. (2005) J Biol Chem 280, 23009-17.
  8. Kim, D.Y. et al. (2005) J Biol Chem 280, 23251-61.
  9. Wallace, R.H. et al. (1998) Nat Genet 19, 366-70.
  10. Lopez-Santiago, L.F. et al. (2007) J Mol Cell Cardiol 43, 636-47.
  11. Chioni, A.M. et al. (2009) Int J Biochem Cell Biol 41, 1216-27.
  12. O'Malley, H.A. et al. (2009) Mol Cell Neurosci 40, 143-55.
  13. Valdivia, C.R. et al. (2010) Cardiovasc Res 86, 392-400.
  14. Schmidt, J.W. and Catterall, W.A. (1986) Cell 46, 437-44.
  15. Watanabe, H. et al. (2009) Circ Arrhythm Electrophysiol 2, 268-75.
  16. Riuró, H. et al. (2013) Hum Mutat 34, 961-6.
  17. Pertin, M. et al. (2005) J Neurosci 25, 10970-80.

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