Product Pathways - Neuroscience
Na Channel β1 Subunit (D4Z2N) Rabbit mAb #13950
|13950S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|13950||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
Na Channel β1 Subunit (D4Z2N) Rabbit mAb recognizes endogenous levels of total sodium channel β1 subunit protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human sodium channel β1 subunit protein.
Western blot analysis of extracts from mouse and rat brain membrane using Na Channel β1 Subunit (D4Z2N) Rabbit mAb.
Mammalian voltage-gated sodium channels (VGSCs) are composed of a pore-forming α subunit and one or more regulatory β subunits (1). Four separate genes (SCN1B-SCN4B) encode the five mammalian β subunits β1, β1B, β2, β3, and β4. In general, β subunit proteins are type I transmembrane proteins, with the exception of secreted β1B protein (reviewed in 2). β subunits regulate α subunit gating and kinetics, which controls cell excitability (3,4). Sodium channel β subunits also function as Ig superfamily cell adhesion molecules that regulate cell adhesion and migration (5,6). Additional research reveals sequential processing of β subunit proteins by β-secretase (BACE1) and γ secretase, resulting in ectodomain shedding of β subunit and generation of an intracellular carboxy-terminal fragment (CTF). Generation of the CTF is thought to play a role in cell adhesion and migration (7,8). Multiple studies demonstrate a link between β subunit gene mutations and a number of disorders, including epilepsy, cardiac arrhythmia, multiple sclerosis, neuropsychiatric disorders, neuropathy, inflammatory pain, and cancer (9-13).
The sodium channel β1 subunit (SCN1B) plays a crucial role in neuronal migration and pathfinding during brain development (14). Mutations in the corresponding SCN1B gene are associated with generalized epilepsy with febrile seizures plus 1 (15), Brugada syndrome (16), and familial atrial fibrillation (17). A SCN1B loss of function mutation results in a severe form of pediatric epileptic encephalopathy known as Dravet syndrome (18).
- Catterall, W.A. (1992) Physiol Rev 72, S15-48.
- Catterall, W.A. (2012) J Physiol 590, 2577-89.
- Isom, L.L. et al. (1992) Science 256, 839-42.
- Brackenbury, W.J. and Isom, L.L. (2011) Front Pharmacol 2, 53.
- Isom, L.L. et al. (1995) Cell 83, 433-42.
- Malhotra, J.D. et al. (2000) J Biol Chem 275, 11383-8.
- Wong, H.K. et al. (2005) J Biol Chem 280, 23009-17.
- Kim, D.Y. et al. (2005) J Biol Chem 280, 23251-61.
- Wallace, R.H. et al. (1998) Nat Genet 19, 366-70.
- Lopez-Santiago, L.F. et al. (2007) J Mol Cell Cardiol 43, 636-47.
- Chioni, A.M. et al. (2009) Int J Biochem Cell Biol 41, 1216-27.
- O'Malley, H.A. et al. (2009) Mol Cell Neurosci 40, 143-55.
- Valdivia, C.R. et al. (2010) Cardiovasc Res 86, 392-400.
- Brackenbury, W.J. et al. (2013) Proc Natl Acad Sci U S A 110, 1089-94.
- Meadows, L.S. et al. (2002) J Neurosci 22, 10699-709.
- Hu, D. et al. (2012) Heart Rhythm 9, 760-9.
- Li, R.G. et al. (2013) Int J Mol Med 32, 144-50.
- Patino, G.A. et al. (2009) J Neurosci 29, 10764-78.
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