Cell Signaling Technology

Product Pathways - Metabolism

Plac8 (E1J2Z) Rabbit mAb #13885

C15   onzin   PLAC8   placenta-specific 8   Placenta-specific gene 8 protein   Protein C15  

No. Size Price
13885S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13885 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 13 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Plac8 (E1J2Z) Rabbit mAb recognizes endogenous levels of total Plac8 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro19 of human Plac8 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Plac8 (E1J2Z) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human Plac8 protein (hPlac8-Myc; +), using Plac8 (E1J2Z) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of Plac8 from LN18 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Plac8 (E1J2Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Plac8 (E1J2Z) Rabbit mAb.

Background

The placenta-specific gene 8 (Plac8) encodes a chromatin-binding protein that was first identified from placenta and that is highly expressed in plasmacytoid dendritic cells (1,2). Research studies indicate that Plac8 plays a role in immune function and is essential in the differentiation of brown fat (3,4). During brown fat differentiation, Plac8 interacts with the transcription factor C/EBPβ at its promoter sequence to activate the transcription of C/EBPβ (4). Experimental deletion of Plac8 in mice leads to impaired innate immune function, abnormal brown fat function, cold intolerance, and obesity (3,4). Additional studies examining the spread of colorectal carcinoma suggest a possible role for Plac8 in cancer invasion, possibly through promoting the epithelial-to-mesenchymal transition seen during tumor progression (5).

  1. Galaviz-Hernandez, C. et al. (2003) Gene 309, 81-9.
  2. Rissoan, M.C. et al. (2002) Blood 100, 3295-303.
  3. Ledford, J.G. et al. (2007) J Immunol 178, 5132-43.
  4. Jimenez-Preitner, M. et al. (2011) Cell Metab 14, 658-70.
  5. Li, C. et al. (2014) J Clin Invest 124, 2172-87.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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