Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

PGRMC1 (D6M5M) XP® Rabbit mAb #13856

PGRMC-1   PGRMC1  

No. Size Price
13856S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
13856T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
13856 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 25 Rabbit IgG
IP 1:50
IHC-P 1:200
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

PGRMC1 (D6M5M) XP® Rabbit mAb recognizes endogenous levels of total PGRMC1 protein. This antibody does not cross-react with PGRMC2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PGRMC1 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293T cells using PGRMC1 (D6M5M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using PGRMC1 (D6M5M) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung non-small cell carcinoma using PGRMC1 (D6M5M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PGRMC1 (D6M5M) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human PGRMC1 protein (hPGRMC1-Myc; +), using PGRMC1 (D6M5M) XP® Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

IP

IP

Immunoprecipitation of PGRMC1 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PGRMC1 (D6M5M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PGRMC1 (D6M5M) XP® Rabbit mAb.

Background

The progesterone receptor membrane component 1 (PGRMC1, Hpr6.6) was originally identified as a component of a progesterone-binding protein complex that also contains plasminogen activator inhibitor 1 RNA binding protein (PAIRBP1, SERBP1) (1,2). The structure of PGRMC1 protein includes a single transmembrane region and a carboxy-terminal cytochrome b5 heme-binding domain (3,4). Research studies confirm that PGRMC1 binds heme as well as binding and regulating cytochrome P450 enzymes responsible for the metabolism of clinical drugs and endogenous signaling molecules (5-7). While early research studies were equivocal on the ability of PGRMC1 to bind progesterone, studies using PGRMC1-fusion proteins clearly demonstrate that PGRMC1 binds progesterone with high affinity (2,8). Studies detailing expression of PGRMC1 in granulosa cells suggest that PGRMC1 mediates the anti-apoptotic actions of progesterone and that this protein is part of a signal transduction pathway that regulates granulosa cell function (9).

  1. Cahill, M.A. (2007) J Steroid Biochem Mol Biol 105, 16-36.
  2. Peluso, J.J. et al. (2008) Endocrinology 149, 534-43.
  3. Gerdes, D. et al. (1998) Biol Chem 379, 907-11.
  4. Mifsud, W. and Bateman, A. (2002) Genome Biol 3, RESEARCH0068.
  5. Crudden, G. et al. (2006) J Pharmacol Exp Ther 316, 448-55.
  6. Hughes, A.L. et al. (2007) Cell Metab 5, 143-9.
  7. Oda, S. et al. (2011) Drug Metab Dispos 39, 2057-65.
  8. Peluso, J.J. et al. (2009) J Clin Endocrinol Metab 94, 2644-9.
  9. Peluso, J.J. (2013) Front Neurosci 7, 99.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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