Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

ERRα (E1G1J) Rabbit mAb #13826

ERR   ERR-1   ERR-alpha   ERR1   ERRalpha   ESRL1   ESRRA   NR3B1   Nuclear Hormone Receptors   Nuclear Receptors   Orphan Nuclear Receptors   sc-32971  

No. Size Price
13826S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
13826 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 50 Rabbit IgG
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig, Horse,

Specificity / Sensitivity

ERRα (E1G1J) Rabbit mAb recognizes endogenous levels of total ERRα protein. This antibody does not cross-react with ERR family members ERRβ and ERRγ, and does not cross-react with either ERα or ERβ.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ERRα protein.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hepa 1-6 cells and either 10 µl of ERRα (E1G1J) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse ESRRA promoter primers, mouse IDH3A intron 2 primers, SimpleChIP® Mouse OGDH Intron 1 Primers #13828, and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ERRα (E1G1J) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from BT-474 cells, untreated (-) or XCT790-treated (5 μM, 24 hr; +), using ERRα (E1G1J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). XCT790 is a selective inverse agonist of ERRα that induces its proteasomal degradation (10,11).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human ERRα protein (hERRα-Myc/DDK; +), full-length human ERRβ protein (hERRβ-Myc/DDK; +); full-length human ERRγ protein (hERRγ-Myc/DDK; +), full-length human ERα protein (ERα-Myc/DDK; +), or full-length human ERβ protein (hERβ-Myc/DDK; +), using ERRα (E1G1J) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).

Western Blotting

Western Blotting

Western blot analysis of heart tissue extracts from ERRα wildtype (+/+) and ERRα knockout (-/-) mice using ERRα (E1G1J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Heart tissue was kindly provided by Dr. Vincent Giguere of McGill University.

Background

The estrogen-related receptor (ERR) subfamily of orphan nuclear receptors include three protein receptors, ERRα/NR3B1, ERRβ/NR3B2, and ERRγ/NR3B3, that have yet to be associated with natural ligands. PGC-1 coactivators regulate ERR transcription activation ability and receptor-induced transcription of genes involved in lipid metabolism, glucose metabolism, and mitochondrial biogenesis (1).

Estrogen-related receptor α (ERRα/NR3B1) is an orphan nuclear receptor that controls transcription of genes involved in fatty acid oxidation, glucose metabolism, and mitochondrial biogenesis (1,2). The receptor protein contains a non-conserved amino terminal domain (NTD), a central zinc finger DNA binding domain, and a ligand-binding domain. The carboxy-terminal AF2 helix motif of ERRα contains binding sites for nuclear receptor coactivators PGC-1α and PGC-1β (3-5). Research studies demonstrate that ERRα transcriptional activity is regulated through phosphorylation and sumoylation within the NTD (6). ERRα is ubiquitously expressed, with strong expression observed in heart, kidneys, skeletal muscle, and other high metabolic demand tissues (2). Additional studies indicate that ERRα is coexpressed in breast tumors with unfavorable biomarkers (7). The pharmacologic inhibition of ERRα activity in breast cancer might serve as a valuable therapeutic approach (8,9).

  1. Giguère, V. (2008) Endocr Rev 29, 677-96.
  2. Giguère, V. et al. (1988) Nature 331, 91-4.
  3. Huss, J.M. et al. (2002) J Biol Chem 277, 40265-74.
  4. Schreiber, S.N. et al. (2003) J Biol Chem 278, 9013-8.
  5. Kamei, Y. et al. (2003) Proc Natl Acad Sci U S A 100, 12378-83.
  6. Tremblay, A.M. et al. (2008) Mol Endocrinol 22, 570-84.
  7. Ariazi, E.A. et al. (2002) Cancer Res 62, 6510-8.
  8. Chang, C.Y. et al. (2011) Cancer Cell 20, 500-10.
  9. Deblois, G. et al. (2009) Cancer Res 69, 6149-57.
  10. Lanvin, O. et al. (2007) J Biol Chem 282, 28328-34.
  11. Willy, P.J. et al. (2004) Proc Natl Acad Sci U S A 101, 8912-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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