Product Pathways - Nuclear Receptor Signaling
ERRα (E1G1J) Rabbit mAb #13826
|13826S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|13826||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, ChIP=Chromatin IP,
Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig, Horse,
Specificity / Sensitivity
ERRα (E1G1J) Rabbit mAb recognizes endogenous levels of total ERRα protein. This antibody does not cross-react with ERR family members ERRβ and ERRγ, and does not cross-react with either ERα or ERβ.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ERRα protein.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hepa 1-6 cells and either 10 µl of ERRα (E1G1J) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using mouse ESRRA promoter primers, mouse IDH3A intron 2 primers, SimpleChIP® Mouse OGDH Intron 1 Primers #13828, and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Western blot analysis of extracts from various cell lines using ERRα (E1G1J) Rabbit mAb.
Western blot analysis of extracts from BT-474 cells, untreated (-) or XCT790-treated (5 μM, 24 hr; +), using ERRα (E1G1J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). XCT790 is a selective inverse agonist of ERRα that induces its proteasomal degradation (10,11).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human ERRα protein (hERRα-Myc/DDK; +), full-length human ERRβ protein (hERRβ-Myc/DDK; +); full-length human ERRγ protein (hERRγ-Myc/DDK; +), full-length human ERα protein (ERα-Myc/DDK; +), or full-length human ERβ protein (hERβ-Myc/DDK; +), using ERRα (E1G1J) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).
The estrogen-related receptor (ERR) subfamily of orphan nuclear receptors include three protein receptors, ERRα/NR3B1, ERRβ/NR3B2, and ERRγ/NR3B3, that have yet to be associated with natural ligands. PGC-1 coactivators regulate ERR transcription activation ability and receptor-induced transcription of genes involved in lipid metabolism, glucose metabolism, and mitochondrial biogenesis (1).
Estrogen-related receptor α (ERRα/NR3B1) is an orphan nuclear receptor that controls transcription of genes involved in fatty acid oxidation, glucose metabolism, and mitochondrial biogenesis (1,2). The receptor protein contains a non-conserved amino terminal domain (NTD), a central zinc finger DNA binding domain, and a ligand-binding domain. The carboxy-terminal AF2 helix motif of ERRα contains binding sites for nuclear receptor coactivators PGC-1α and PGC-1β (3-5). Research studies demonstrate that ERRα transcriptional activity is regulated through phosphorylation and sumoylation within the NTD (6). ERRα is ubiquitously expressed, with strong expression observed in heart, kidneys, skeletal muscle, and other high metabolic demand tissues (2). Additional studies indicate that ERRα is coexpressed in breast tumors with unfavorable biomarkers (7). The pharmacologic inhibition of ERRα activity in breast cancer might serve as a valuable therapeutic approach (8,9).
- Giguère, V. (2008) Endocr Rev 29, 677-96.
- Giguère, V. et al. (1988) Nature 331, 91-4.
- Huss, J.M. et al. (2002) J Biol Chem 277, 40265-74.
- Schreiber, S.N. et al. (2003) J Biol Chem 278, 9013-8.
- Kamei, Y. et al. (2003) Proc Natl Acad Sci U S A 100, 12378-83.
- Tremblay, A.M. et al. (2008) Mol Endocrinol 22, 570-84.
- Ariazi, E.A. et al. (2002) Cancer Res 62, 6510-8.
- Chang, C.Y. et al. (2011) Cancer Cell 20, 500-10.
- Deblois, G. et al. (2009) Cancer Res 69, 6149-57.
- Lanvin, O. et al. (2007) J Biol Chem 282, 28328-34.
- Willy, P.J. et al. (2004) Proc Natl Acad Sci U S A 101, 8912-7.
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- 9006 ChIP-Grade Protein G Magnetic Beads
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- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
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